Display options
Cite
Smolarek S, Vdovin A, Perrier DL, et al. High-resolution excitation and absorption spectroscopy of gas-phase p-coumaric acid: unveiling an elusive chromophore. J Am Chem Soc. 2010;132(18):6315-7doi: 10.1021/ja101668v.
Smolarek, S., Vdovin, A., Perrier, D. L., Smit, J. P., Drabbels, M., & Buma, W. J. (2010). High-resolution excitation and absorption spectroscopy of gas-phase p-coumaric acid: unveiling an elusive chromophore. Journal of the American Chemical Society, 132(18), 6315-7. https://doi.org/10.1021/ja101668v
Smolarek, Szymon, et al. "High-resolution excitation and absorption spectroscopy of gas-phase p-coumaric acid: unveiling an elusive chromophore." Journal of the American Chemical Society vol. 132,18 (2010): 6315-7. doi: https://doi.org/10.1021/ja101668v
Smolarek S, Vdovin A, Perrier DL, Smit JP, Drabbels M, Buma WJ. High-resolution excitation and absorption spectroscopy of gas-phase p-coumaric acid: unveiling an elusive chromophore. J Am Chem Soc. 2010 May 12;132(18):6315-7. doi: 10.1021/ja101668v. PMID: 20397707.
Copy Download .nbib Format: NLM
Share it on

J Am Chem Soc. 2010 May 12;132(18):6315-7. doi: 10.1021/ja101668v.

High-resolution excitation and absorption spectroscopy of gas-phase p-coumaric acid: unveiling an elusive chromophore.

Journal of the American Chemical Society

Szymon Smolarek, Alexander Vdovin, Dayinta L Perrier, Jorrit P Smit, Marcel Drabbels, Wybren J Buma

Affiliations

  1. University of Amsterdam, Nieuwe Achtergracht 166, 1018 WV Amsterdam, The Netherlands.

PMID: 20397707 DOI: 10.1021/ja101668v

Abstract

We report on the first successful high-resolution spectroscopic studies on isolated para-coumaric acid, the chromophore of the photoactive yellow protein which has become a model system for studying biological light-induced signal transduction. Employing various double-resonance multiphoton ionization techniques in combination with mass-resolved ion detection and the results of quantum chemical calculations, we identify three conformations the molecule can adopt under our experimental conditions. The vibrational activity in the excitation spectra allows us to conclude that in the Franck-Condon region accessed from the ground state S(1) is the V'(pipi*) state. Interestingly, we find considerable out-of-plane vibrational activity, indicating that the molecule adopts a nonplanar geometry in S(1). The ionization requirements show that after excitation rapid internal conversion takes place to a lower-lying npi* state. Such a state has been postulated by ab initio calculations on para-coumaric acid and derivatives, but until the present study no direct evidence had been found for its presence.

Publication Types