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Mulder AH, van Amsterdam RG, Wilbrink M, et al. Depolarization-induced release of [(3)H]histamine by high potassium concentrations, electrical stimulation and veratrine from rat brain slices after incubation with the radiolabelled amine. Neurochem Int. 1983;5(3):291-7doi: 10.1016/0197-0186(83)90031-1.
Mulder, A. H., van Amsterdam, R. G., Wilbrink, M., & Schoffelmeer, A. N. (1983). Depolarization-induced release of [(3)H]histamine by high potassium concentrations, electrical stimulation and veratrine from rat brain slices after incubation with the radiolabelled amine. Neurochemistry international, 5(3), 291-7. https://doi.org/10.1016/0197-0186(83)90031-1
Mulder, A H, et al. "Depolarization-induced release of [(3)H]histamine by high potassium concentrations, electrical stimulation and veratrine from rat brain slices after incubation with the radiolabelled amine." Neurochemistry international vol. 5,3 (1983): 291-7. doi: https://doi.org/10.1016/0197-0186(83)90031-1
Mulder AH, van Amsterdam RG, Wilbrink M, Schoffelmeer AN. Depolarization-induced release of [(3)H]histamine by high potassium concentrations, electrical stimulation and veratrine from rat brain slices after incubation with the radiolabelled amine. Neurochem Int. 1983;5(3):291-7. doi: 10.1016/0197-0186(83)90031-1. PMID: 20487952.
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Neurochem Int. 1983;5(3):291-7. doi: 10.1016/0197-0186(83)90031-1.

Depolarization-induced release of [(3)H]histamine by high potassium concentrations, electrical stimulation and veratrine from rat brain slices after incubation with the radiolabelled amine.

Neurochemistry international

A H Mulder, R G van Amsterdam, M Wilbrink, A N Schoffelmeer

Affiliations

  1. Department of Pharmacology, Free University, Medical Faculty Van der Boechorststraat 7, 1081 BT Amsterdam, The Netherlands.

PMID: 20487952 DOI: 10.1016/0197-0186(83)90031-1

Abstract

Brain slices obtained from neocortex, hypothalamus or hippocampus were incubated with [(3)H]histamine and subsequently superfused and exposed to different depolarizing stimuli, viz. high K(+)-concentrations, electrical field stimulation and veratrine. K(+)-induced release of tritium was completely calcium-dependent and its magnitude depended on the K(+)-concentration, with maximal release being reached at 56 mM K(+). Electrically-evoked release of tritium increased with increasing frequencies and reached its maximum at about 20 Hz. The electrically-evoked release appeared to be totally calcium-dependent and it was strongly inhibited by tetrodotoxin. Veratrine (5-100 ?M) also induced a release of tritium; maximal release was obtained at 100 ?M veratrine. Veratrine-induced release was partially calcium-dependent and was strongly reduced by tetrodotoxin. Taken together the data indicate that the depolarization-induced release of tritium from brain slices pre-labelled with [(3)H]histamine, represents [(3)H]histamine release from neurons and not from either mast cells or glial cells. It remains to be established whether these neurons are specifically histaminergic.

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