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Boyd PJ, Osborne NN, Walker RJ. The pharmacological actions of 5-hydroxytryptamine, FMRF-amide and substance P and their possible occurrence in the heart of the snail Helix aspersa L. Neurochem Int. 1984;6(5):633-40doi: 10.1016/0197-0186(84)90044-5.
Boyd, P. J., Osborne, N. N., & Walker, R. J. (1984). The pharmacological actions of 5-hydroxytryptamine, FMRF-amide and substance P and their possible occurrence in the heart of the snail Helix aspersa L. Neurochemistry international, 6(5), 633-40. https://doi.org/10.1016/0197-0186(84)90044-5
Boyd, P J, et al. "The pharmacological actions of 5-hydroxytryptamine, FMRF-amide and substance P and their possible occurrence in the heart of the snail Helix aspersa L." Neurochemistry international vol. 6,5 (1984): 633-40. doi: https://doi.org/10.1016/0197-0186(84)90044-5
Boyd PJ, Osborne NN, Walker RJ. The pharmacological actions of 5-hydroxytryptamine, FMRF-amide and substance P and their possible occurrence in the heart of the snail Helix aspersa L. Neurochem Int. 1984;6(5):633-40. doi: 10.1016/0197-0186(84)90044-5. PMID: 20488089.
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Neurochem Int. 1984;6(5):633-40. doi: 10.1016/0197-0186(84)90044-5.

The pharmacological actions of 5-hydroxytryptamine, FMRF-amide and substance P and their possible occurrence in the heart of the snail Helix aspersa L.

Neurochemistry international

P J Boyd, N N Osborne, R J Walker

Affiliations

  1. Department of Neurophysiology, School of Biochemical and Physiological Sciences, University Southampton, Southampton SO9 3TU, U.K.

PMID: 20488089 DOI: 10.1016/0197-0186(84)90044-5

Abstract

The actions of 5-hydroxytryptamine (5-HT), FMRF-amide and substance P were tested on the isolated heart of Helix aspersa. All three compounds were found to produce positive inotropic and chronotropic effects, the order of potency being 5-HT > substance P > FMRF-amide. However, whereas the responses to 5-HT and FMRF-amide were maximal within a few seconds, the response to substance P had a longer latency. Two other similar undecapeptides, eledoisin and physalaemin, were also tested and were found to have very similar actions to those of substance P. The effects of 5-HT and FMRF-amide could be separated using the 5-HT blockers methysergide and ketanserin, which had relatively little effect on the response to FMRF-amide. Immunohistochemical staining was carried out on sections through the Helix auricle and ventricle for 5-HT, FMRF-amide and substance P. Substantial 5-HT-like and substance P-like immunoreactivity was observed, apparently concentrated in nerve endings, but the level of FMRF-amide-like immunoreactivity was considerably lower. The fluorescence produced by all three compounds was evenly distributed throughout the heart tissue. HPLC analysis of heart tissue extract demonstrated a high level of 5-HT (about 8 ?g/g wet weight) but a negligible catecholamine content.

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