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Gehlert DR, Wamsley JK. Dopamine receptors in the rat brain: quantitative autoradiographic localization using [(3)H]sulpiride. Neurochem Int. 1985;7(4):717-23doi: 10.1016/0197-0186(85)90070-1.
Gehlert, D. R., & Wamsley, J. K. (1985). Dopamine receptors in the rat brain: quantitative autoradiographic localization using [(3)H]sulpiride. Neurochemistry international, 7(4), 717-23. https://doi.org/10.1016/0197-0186(85)90070-1
Gehlert, D R, and Wamsley, J K. "Dopamine receptors in the rat brain: quantitative autoradiographic localization using [(3)H]sulpiride." Neurochemistry international vol. 7,4 (1985): 717-23. doi: https://doi.org/10.1016/0197-0186(85)90070-1
Gehlert DR, Wamsley JK. Dopamine receptors in the rat brain: quantitative autoradiographic localization using [(3)H]sulpiride. Neurochem Int. 1985;7(4):717-23. doi: 10.1016/0197-0186(85)90070-1. PMID: 20492979.
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Neurochem Int. 1985;7(4):717-23. doi: 10.1016/0197-0186(85)90070-1.

Dopamine receptors in the rat brain: quantitative autoradiographic localization using [(3)H]sulpiride.

Neurochemistry international

D R Gehlert, J K Wamsley

Affiliations

  1. Departments of Psychiatry and Pharmacology, University of Utah School of Medicine, Salt Lake City, UT 84132, U.S.A.

PMID: 20492979 DOI: 10.1016/0197-0186(85)90070-1

Abstract

In vitro labeling of tissue sections with [(3)H]sulpiride has been utilized in the present study to autoradiographically localize D(2)-dopamine receptors in the rat brain. Preliminary biochemical studies, using slide-mounted tissue sections, were performed to define the optimal labeling conditions for this binding. Autoradiograms were generated by apposition of the labeled tissue sections to tritium-sensitive film. Specific binding sites for [(3)H]sulpiride were localized to the caudate-putamen, nucleus accumbens, olfactory tubercle, glomerular layer of the olfactory bulb, pituitary, laminae I and III of the entorhinal cortex, substantia nigra, lateral mammillary nucleus and the stratum-lacunosum moleculare of the hippocampus. The high selectivity of [(3)H]sulpiride for the D(2)-dopamine receptor indicates that it is a valuable tool for the autoradiographic localization and quantitation of neuroleptic receptors.

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