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Motari E, Zheng X, Su X, et al. Analysis of Recombinant CD24 Glycans by MALDI-TOF-MS Reveals Prevalence of Sialyl-T Antigen. Am J Biomed Sci. 2009;1(1):1-11doi: 10.5099/aj090100001.
Motari, E., Zheng, X., Su, X., Liu, Y., Kvaratskhelia, M., Freitas, M., & Wang, P. G. (2009). Analysis of Recombinant CD24 Glycans by MALDI-TOF-MS Reveals Prevalence of Sialyl-T Antigen. American journal of biomedical sciences, 1(1), 1-11. https://doi.org/10.5099/aj090100001
Motari, Edwin, et al. "Analysis of Recombinant CD24 Glycans by MALDI-TOF-MS Reveals Prevalence of Sialyl-T Antigen." American journal of biomedical sciences vol. 1,1 (2009): 1-11. doi: https://doi.org/10.5099/aj090100001
Motari E, Zheng X, Su X, Liu Y, Kvaratskhelia M, Freitas M, Wang PG. Analysis of Recombinant CD24 Glycans by MALDI-TOF-MS Reveals Prevalence of Sialyl-T Antigen. Am J Biomed Sci. 2009 Jan 01;1(1):1-11. doi: 10.5099/aj090100001. PMID: 20407596; PMCID: PMC2856090.
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Am J Biomed Sci. 2009 Jan 01;1(1):1-11. doi: 10.5099/aj090100001.

Analysis of Recombinant CD24 Glycans by MALDI-TOF-MS Reveals Prevalence of Sialyl-T Antigen.

American journal of biomedical sciences

Edwin Motari, Xincheng Zheng, Xiaodan Su, Yang Liu, Mamuka Kvaratskhelia, Michael Freitas, Peng George Wang

Affiliations

  1. Departments of Chemistry and Biochemistry, The Ohio State University, Columbus, OH 43210, USA.

PMID: 20407596 PMCID: PMC2856090 DOI: 10.5099/aj090100001

Abstract

CD24 is a glycosyl-phosphatidyl-inositol linked glycoprotein expressed in a broad range of cell types and is heavily glycosylated. It has been found to be over expressed in cancers and tumors and is also a costimulatory molecule. Therefore, this study was carried out to define the structures of the carbohydrates associated with the CD24 recombinant protein. The CD24 glycoprotein's oligosaccharides were released by chemical and enzymatic means prior to being analyzed by MALDI-TOF-MS. The results obtained showed that CD24 is both N- and O-glycosylated. The major oligosaccharides were found to be Neu5Acα-2,3/6Galβ-1,3GalNAc, NeuAc(2)Gal β-1,3GalNAc(1) (O-glycans), GalNAc(2)GlcNAc(2)Man(3)Fuc(1), Gal(1)GalNAc(2)GlcNAc(2)Man(3)Fuc(1), and Gal(2)GalNAc(2)GlcNAc(2)Man(3)Fuc(1) (N-glycans). The results showed that Neu5Acα-2,3/6Galβ-1,3GalNAc (sialyl-tumor antigen, sT), a cancer-associated carbohydrate, was the most abundant glycan associated with CD24. This result raised the intriguing possibility that CD24 may be a major carrier of the sialyl-T abundantly found in cancer cells.

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