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Agarwal S, Gopal K, Chhabra G, et al. Molecular cloning, sequence analysis and homology modeling of galE encoding UDP-galactose 4-epimerase of Aeromonas hydrophila. Bioinformation. 2009;4(5):216-22doi: 10.6026/97320630004216.
Agarwal, S., Gopal, K., Chhabra, G., & Dixit, A. (2009). Molecular cloning, sequence analysis and homology modeling of galE encoding UDP-galactose 4-epimerase of Aeromonas hydrophila. Bioinformation, 4(5), 216-22. https://doi.org/10.6026/97320630004216
Agarwal, Shivani, et al. "Molecular cloning, sequence analysis and homology modeling of galE encoding UDP-galactose 4-epimerase of Aeromonas hydrophila." Bioinformation vol. 4,5 (2009): 216-22. doi: https://doi.org/10.6026/97320630004216
Agarwal S, Gopal K, Chhabra G, Dixit A. Molecular cloning, sequence analysis and homology modeling of galE encoding UDP-galactose 4-epimerase of Aeromonas hydrophila. Bioinformation. 2009 Nov 17;4(5):216-22. doi: 10.6026/97320630004216. PMID: 20461162; PMCID: PMC2859578.
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Bioinformation. 2009 Nov 17;4(5):216-22. doi: 10.6026/97320630004216.

Molecular cloning, sequence analysis and homology modeling of galE encoding UDP-galactose 4-epimerase of Aeromonas hydrophila.

Bioinformation

Shivani Agarwal, Keshav Gopal, Gagan Chhabra, Aparna Dixit

Affiliations

  1. Gene Regulation Laboratory, School of Biotechnology, Jawaharlal Nehru University, New Delhi - 110067, India.

PMID: 20461162 PMCID: PMC2859578 DOI: 10.6026/97320630004216

Abstract

A. hydrophila, a ubiquitous gram-negative bacterium present in aquatic environments, has been implicated in illness in humans, fish and amphibians. Lipopolysaccharides (LPS), a surface component of the outer membrane, are one of the main virulent factors of gram-negative bacteria. UDP-galactose 4-epimerase (GalE) catalyses the last step in the Leloir pathway of galactose metabolism and provides precursor for the biosynthesis of extracellular LPS and capsule. Due to its key role in LPS biosynthesis, it is a potential drug target. The present study describes cloning, sequence analysis and prediction of three dimensional structure of the deduced amino acid sequence of the galE of A. hydrophila AH17. The cloned galE consists of the putative promoter-operator region, and an open reading frame of 338 amino acid residues. Sequence alignment and predicted 3Dstructure revealed that the GalE of A. hydrophila consists of the signature sequences of the epimerase super family. The present study reports the molecular modeling / 3D-structure prediction of GalE of A. hydrophila. Further, the potential regions of the enzyme that can be targeted for drug design are identified.

Keywords: Aeromonas hydrophila; UDP-galactose 4- epimerase; lipopolysaccharide; molecular phylogenetic; virulence

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