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Gullo F, Mazzetti S, Maffezzoli A, et al. Orchestration of "presto" and "largo" synchrony in up-down activity of cortical networks. Front Neural Circuits. 2010;4:11doi: 10.3389/fncir.2010.00011.
Gullo, F., Mazzetti, S., Maffezzoli, A., Dossi, E., Lecchi, M., Amadeo, A., Krajewski, J., & Wanke, E. (2010). Orchestration of "presto" and "largo" synchrony in up-down activity of cortical networks. Frontiers in neural circuits, 411. https://doi.org/10.3389/fncir.2010.00011
Gullo, Francesca, et al. "Orchestration of "presto" and "largo" synchrony in up-down activity of cortical networks." Frontiers in neural circuits vol. 4 (2010): 11. doi: https://doi.org/10.3389/fncir.2010.00011
Gullo F, Mazzetti S, Maffezzoli A, Dossi E, Lecchi M, Amadeo A, Krajewski J, Wanke E. Orchestration of "presto" and "largo" synchrony in up-down activity of cortical networks. Front Neural Circuits. 2010 Apr 22;4:11. doi: 10.3389/fncir.2010.00011. eCollection 2010. PMID: 20461235; PMCID: PMC2866559.
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Front Neural Circuits. 2010 Apr 22;4:11. doi: 10.3389/fncir.2010.00011. eCollection 2010.

Orchestration of "presto" and "largo" synchrony in up-down activity of cortical networks.

Frontiers in neural circuits

Francesca Gullo, Samanta Mazzetti, Andrea Maffezzoli, Elena Dossi, Marzia Lecchi, Alida Amadeo, Jeffrey Krajewski, Enzo Wanke

Affiliations

  1. Department of Biotechnologies and Biosciences, University of Milano-Bicocca Milan, Italy.

PMID: 20461235 PMCID: PMC2866559 DOI: 10.3389/fncir.2010.00011

Abstract

It has been demonstrated using single-cell and multiunit electrophysiology in layer III entorhinal cortex and disinhibited hippocampal CA3 slices that the balancing of the up-down activity is characterized by both GABA(A) and GABA(B) mechanisms. Here we report novel results obtained using multi-electrode array (60 electrodes) simultaneous recordings from reverberating postnatal neocortical networks containing 19.2 +/- 1.4% GABAergic neurons, typical of intact tissue. We observed that in each spontaneous active-state the total number of spikes in identified clusters of excitatory and inhibitory neurons is almost equal, thus suggesting a balanced average activity. Interestingly, in the active-state, the early phase is sustained by only 10% of the total spikes and the firing rate follows a sigmoidal regenerative mode up to peak at 35 ms with the number of excitatory spikes greater than inhibitory, therefore indicating an early unbalance. Concentration-response pharmacology of up- and down-state lifetimes in clusters of excitatory (n = 1067) and inhibitory (n = 305) cells suggests that, besides the GABA(A) and GABA(B) mechanisms, others such as GAT-1-mediated uptake, I(h), I(NaP) and I(M) ion channel activity, robustly govern both up- and down-activity. Some drugs resulted to affect up- and/or down-states with different IC(50)s, providing evidence that various mechanisms are involved. These results should reinforce not only the role of synchrony in CNS networks, but also the recognized analogies between the Hodgkin-Huxley action potential and the population bursts as basic mechanisms for originating membrane excitability and CNS network synchronization, respectively.

Keywords: GABA; IM channels; INaP channels; Ih channels; MEA recording; bursts; synchrony

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