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Schmidt M, Wolfram T, Rumpler M, et al. Live cell adhesion assay with attenuated total reflection infrared spectroscopy. Biointerphases. 2007;2(1):1-5doi: 10.1116/1.2710336.
Schmidt, M., Wolfram, T., Rumpler, M., Tripp, C. P., & Grunze, M. (2007). Live cell adhesion assay with attenuated total reflection infrared spectroscopy. Biointerphases, 2(1), 1-5. https://doi.org/10.1116/1.2710336
Schmidt, Martin, et al. "Live cell adhesion assay with attenuated total reflection infrared spectroscopy." Biointerphases vol. 2,1 (2007): 1-5. doi: https://doi.org/10.1116/1.2710336
Schmidt M, Wolfram T, Rumpler M, Tripp CP, Grunze M. Live cell adhesion assay with attenuated total reflection infrared spectroscopy. Biointerphases. 2007 Mar;2(1):1-5. doi: 10.1116/1.2710336. PMID: 20408630.
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Biointerphases. 2007 Mar;2(1):1-5. doi: 10.1116/1.2710336.

Live cell adhesion assay with attenuated total reflection infrared spectroscopy.

Biointerphases

Martin Schmidt, Tobias Wolfram, Monika Rumpler, Carl P Tripp, Michael Grunze

Affiliations

  1. Institute for Molecular Biophysics, Bar Harbor, Maine 04609, USA. [email protected]

PMID: 20408630 DOI: 10.1116/1.2710336

Abstract

Living confluent fish fibroblast cells RTG-P1 from rainbow trout adherent on diamond were examined by attenuated total reflection (ATR) infrared (IR) spectroscopy. In particular, IR spectra were recorded dynamically during the adsorption of the cells onto the diamond and during their biochemically induced structural responses to the subsequent addition of trypsin and cytochalasin D. It is shown that changes in the IR spectra result from changes in cell morphology and surface coverage. The results demonstrate the potential and the applicability of ATR IR spectroscopy for live cell adhesion assays.

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