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Deacon CF, Conlon JM. Biosynthesis of tachykinins (substance P, neurokinin A and neuropeptide K) in neurons of the guinea pig myenteric plexus. Neurochem Int. 1987;10(4):559-64doi: 10.1016/0197-0186(87)90085-4.
Deacon, C. F., & Conlon, J. M. (1987). Biosynthesis of tachykinins (substance P, neurokinin A and neuropeptide K) in neurons of the guinea pig myenteric plexus. Neurochemistry international, 10(4), 559-64. https://doi.org/10.1016/0197-0186(87)90085-4
Deacon, C F, and Conlon, J M. "Biosynthesis of tachykinins (substance P, neurokinin A and neuropeptide K) in neurons of the guinea pig myenteric plexus." Neurochemistry international vol. 10,4 (1987): 559-64. doi: https://doi.org/10.1016/0197-0186(87)90085-4
Deacon CF, Conlon JM. Biosynthesis of tachykinins (substance P, neurokinin A and neuropeptide K) in neurons of the guinea pig myenteric plexus. Neurochem Int. 1987;10(4):559-64. doi: 10.1016/0197-0186(87)90085-4. PMID: 20501131.
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Neurochem Int. 1987;10(4):559-64. doi: 10.1016/0197-0186(87)90085-4.

Biosynthesis of tachykinins (substance P, neurokinin A and neuropeptide K) in neurons of the guinea pig myenteric plexus.

Neurochemistry international

C F Deacon, J M Conlon

Affiliations

  1. Clinical Research Group for Gastrointestinal Endocrinology, Max-Planck-Gesellschaft, University of Göttingen, Gosslerstrasse 10D, D-3400 Göttingen, F.R.G.

PMID: 20501131 DOI: 10.1016/0197-0186(87)90085-4

Abstract

Incubation of the myenteric plexus-containing longitudinal muscle layer of guinea pig small intestine with [(35)S]methionine for up to 9 h resulted in a progressive increase in the incorporation of radioactivity into newly synthesized substance P, neurokinin A and neuropeptide K. The radiolabelled peptides were isolated from tissue extracts by immunoprecipitation using regionally-specific antisera and purified by reverse-phase high performance liquid chromatography. Incorporation of radioactivity into the tachykinins was abolished by cycloheximide and was not observed when [(35)S]cysteine was substituted for [(35)S]methionine. The method may be used to study the regulation of biosynthesis and posttranslational modification of protachykinins in the gut.

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