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Santer DM, Heydorn WE, Creed GJ, et al. Localization of Ca(2+)-binding proteins of rat cortex on two-dimensional gels-II. analysis of Ca(2+) binding proteins in ammonium sulfate fractions of rat brain. Neurochem Int. 1988;12(2):225-36doi: 10.1016/0197-0186(88)90131-3.
Santer, D. M., Heydorn, W. E., Creed, G. J., Fukuda, T., & Jacobowitz, D. M. (1988). Localization of Ca(2+)-binding proteins of rat cortex on two-dimensional gels-II. analysis of Ca(2+) binding proteins in ammonium sulfate fractions of rat brain. Neurochemistry international, 12(2), 225-36. https://doi.org/10.1016/0197-0186(88)90131-3
Santer, D M, et al. "Localization of Ca(2+)-binding proteins of rat cortex on two-dimensional gels-II. analysis of Ca(2+) binding proteins in ammonium sulfate fractions of rat brain." Neurochemistry international vol. 12,2 (1988): 225-36. doi: https://doi.org/10.1016/0197-0186(88)90131-3
Santer DM, Heydorn WE, Creed GJ, Fukuda T, Jacobowitz DM. Localization of Ca(2+)-binding proteins of rat cortex on two-dimensional gels-II. analysis of Ca(2+) binding proteins in ammonium sulfate fractions of rat brain. Neurochem Int. 1988;12(2):225-36. doi: 10.1016/0197-0186(88)90131-3. PMID: 20501225.
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Neurochem Int. 1988;12(2):225-36. doi: 10.1016/0197-0186(88)90131-3.

Localization of Ca(2+)-binding proteins of rat cortex on two-dimensional gels-II. analysis of Ca(2+) binding proteins in ammonium sulfate fractions of rat brain.

Neurochemistry international

D M Santer, W E Heydorn, G J Creed, T Fukuda, D M Jacobowitz

Affiliations

  1. Laboratory of Clinical Science, National Institute of Mental Health, Bethesda, MD 20892, U.S.A.

PMID: 20501225 DOI: 10.1016/0197-0186(88)90131-3

Abstract

A total of seven high-affinity calcium-binding proteins have been detected in rat brain. This was accomplished using a combination of ammonium sulfate fractionation, two-dimensional gel electrophoresis, western blotting and (45)Ca(2+)-autoradiography. Of these seven proteins, three are detectable in a crude tissue punch of rat cortex while four are seen only after protein enrichment with ammonium sulfate. Four of the seven proteins detected in this study have been identified: calmodulin, the B subunit of calcineurin, the intestinal vitamin D-dependent calcium-binding protein and parvalbumin. The identities of the other three proteins visualized by (45)Ca(2+)-autoradiography in this study are unknown.

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