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Martini C, Pacini R, Lucacchini A. Inactivation of [(35)S]t-butylbicyclophosphorothionate binding sites by the arginine reagent, 2,3-butanedione. Neurochem Int. 1991;18(1):51-4doi: 10.1016/0197-0186(91)90035-c.
Martini, C., Pacini, R., & Lucacchini, A. (1991). Inactivation of [(35)S]t-butylbicyclophosphorothionate binding sites by the arginine reagent, 2,3-butanedione. Neurochemistry international, 18(1), 51-4. https://doi.org/10.1016/0197-0186(91)90035-c
Martini, C, et al. "Inactivation of [(35)S]t-butylbicyclophosphorothionate binding sites by the arginine reagent, 2,3-butanedione." Neurochemistry international vol. 18,1 (1991): 51-4. doi: https://doi.org/10.1016/0197-0186(91)90035-c
Martini C, Pacini R, Lucacchini A. Inactivation of [(35)S]t-butylbicyclophosphorothionate binding sites by the arginine reagent, 2,3-butanedione. Neurochem Int. 1991;18(1):51-4. doi: 10.1016/0197-0186(91)90035-c. PMID: 20504675.
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Neurochem Int. 1991;18(1):51-4. doi: 10.1016/0197-0186(91)90035-c.

Inactivation of [(35)S]t-butylbicyclophosphorothionate binding sites by the arginine reagent, 2,3-butanedione.

Neurochemistry international

C Martini, R Pacini, A Lucacchini

Affiliations

  1. Istituto Policattedra di Discipline Biologiche, Università di Pisa, Via Bonanno, 56100 Pisa, Italia; Istituto di Chimica Biologica, Università di Parma, 43100 Parma, Italia.

PMID: 20504675 DOI: 10.1016/0197-0186(91)90035-c

Abstract

The "cage" convulsant [(35)S]t-butylbicyclophosphorothionate ([(35)S]TBPS) binds with high affinity to specific sites "at" or "near" a ?-amino-butyric acid (GABA)-gated chloride channel according to current hypothesis. We now report that pretreatment of membranes with 2,3-butanedione, an arginine-specific reagent, causes a dose- and time-dependent decrease in the number of [(35)S]TBPS binding sites. No decrease occurs when membranes are pretreated with 2,3-butanedione in the presence of picrotoxinin. Binding of [(35)S]TBPS to the remaining sites occurs with the same characteristics as binding to the untreated receptor population.

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