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Wang P, Robert L, Pelletier J, et al. Robust growth of Escherichia coli. Curr Biol. 2010;20(12):1099-103doi: 10.1016/j.cub.2010.04.045.
Wang, P., Robert, L., Pelletier, J., Dang, W. L., Taddei, F., Wright, A., & Jun, S. (2010). Robust growth of Escherichia coli. Current biology : CB, 20(12), 1099-103. https://doi.org/10.1016/j.cub.2010.04.045
Wang, Ping, et al. "Robust growth of Escherichia coli." Current biology : CB vol. 20,12 (2010): 1099-103. doi: https://doi.org/10.1016/j.cub.2010.04.045
Wang P, Robert L, Pelletier J, Dang WL, Taddei F, Wright A, Jun S. Robust growth of Escherichia coli. Curr Biol. 2010 Jun 22;20(12):1099-103. doi: 10.1016/j.cub.2010.04.045. Epub 2010 May 27. PMID: 20537537; PMCID: PMC2902570.
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Curr Biol. 2010 Jun 22;20(12):1099-103. doi: 10.1016/j.cub.2010.04.045. Epub 2010 May 27.

Robust growth of Escherichia coli.

Current biology : CB

Ping Wang, Lydia Robert, James Pelletier, Wei Lien Dang, Francois Taddei, Andrew Wright, Suckjoon Jun

Affiliations

  1. FAS Center for Systems Biology, Harvard University, 52 Oxford St, Cambridge, MA 02138, USA.

PMID: 20537537 PMCID: PMC2902570 DOI: 10.1016/j.cub.2010.04.045

Abstract

The quantitative study of the cell growth has led to many fundamental insights in our understanding of a wide range of subjects, from the cell cycle to senescence. Of particular importance is the growth rate, whose constancy represents a physiological steady state of an organism. Recent studies, however, suggest that the rate of elongation during exponential growth of bacterial cells decreases cumulatively with replicative age for both asymmetrically and symmetrically dividing organisms, implying that a "steady-state" population consists of individual cells that are never in a steady state of growth. To resolve this seeming paradoxical observation, we studied the long-term growth and division patterns of Escherichia coli cells by employing a microfluidic device designed to follow steady-state growth and division of a large number of cells at a defined reproductive age. Our analysis of approximately 10(5) individual cells reveals a remarkable stability of growth whereby the mother cell inherits the same pole for hundreds of generations. We further show that death of E. coli is not purely stochastic but is the result of accumulating damages. We conclude that E. coli, unlike all other aging model systems studied to date, has a robust mechanism of growth that is decoupled from cell death.

Copyright 2010 Elsevier Ltd. All rights reserved.

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