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Trump BF, Berezesky IK, Smith MW, et al. Studies of human bronchial epithelium in vitro: Changes of [Ca(2+)](i) in relation to injury, growth and differentiation. Toxicol In Vitro. 1990;4(4):646-53doi: 10.1016/0887-2333(90)90135-g.
Trump, B. F., Berezesky, I. K., Smith, M. W., Jones, R. T., Phelps, P. C., Miyashita, M., & Harris, C. C. (1990). Studies of human bronchial epithelium in vitro: Changes of [Ca(2+)](i) in relation to injury, growth and differentiation. Toxicology in vitro : an international journal published in association with BIBRA, 4(4), 646-53. https://doi.org/10.1016/0887-2333(90)90135-g
Trump, B F, et al. "Studies of human bronchial epithelium in vitro: Changes of [Ca(2+)](i) in relation to injury, growth and differentiation." Toxicology in vitro : an international journal published in association with BIBRA vol. 4,4 (1990): 646-53. doi: https://doi.org/10.1016/0887-2333(90)90135-g
Trump BF, Berezesky IK, Smith MW, Jones RT, Phelps PC, Miyashita M, Harris CC. Studies of human bronchial epithelium in vitro: Changes of [Ca(2+)](i) in relation to injury, growth and differentiation. Toxicol In Vitro. 1990;4(4):646-53. doi: 10.1016/0887-2333(90)90135-g. PMID: 20702249.
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Toxicol In Vitro. 1990;4(4):646-53. doi: 10.1016/0887-2333(90)90135-g.

Studies of human bronchial epithelium in vitro: Changes of [Ca(2+)](i) in relation to injury, growth and differentiation.

Toxicology in vitro : an international journal published in association with BIBRA

B F Trump, I K Berezesky, M W Smith, R T Jones, P C Phelps, M Miyashita, C C Harris

Affiliations

  1. Department of Pathology, University of Maryland School of Medicine, USA; Maryland Institute for Emergency Medical Services Systems, Baltimore, MD 21201, USA.

PMID: 20702249 DOI: 10.1016/0887-2333(90)90135-g

Abstract

The objectives of the present work included the study of the response of normal human bronchial epithelial (NHBE) cells to injury following treatment with several agents, especially those associated with tumour promotion. The effects of putative tumour promoters including 12-O-tetradecanoylphorbol acetate (TPA), a series of aldehydes, and H(2)O(2) on the regulation of cytosolic ionized calcium ([Ca(2+)](i)) were compared with the effects of growth and squamous differentiation stimuli, including serum and transforming growth factor-beta. The cells studied included primary cultures of NHBE cells and the BEAS-2B cell line, created by transformation of NHBE cells with the adenovirus 12-Simian virus 40 hybrid. The results indicate that, with several stimuli, the onset of terminal squamous differentiation is preceded by a rise in [Ca(2+)](i). The results are interpreted in terms of a general hypothesis regarding the interaction between acute and chronic cell injury.

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