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Imreh G, Beckman M, Iverfeldt K, et al. Development of Non-invasive Methods to Study Cell Death. Toxicol In Vitro. 1998;12(5):561-5doi: 10.1016/s0887-2333(98)00036-8.
Imreh, G., Beckman, M., Iverfeldt, K., & Hallberg, E. (1998). Development of Non-invasive Methods to Study Cell Death. Toxicology in vitro : an international journal published in association with BIBRA, 12(5), 561-5. https://doi.org/10.1016/s0887-2333(98)00036-8
Imreh, G, et al. "Development of Non-invasive Methods to Study Cell Death." Toxicology in vitro : an international journal published in association with BIBRA vol. 12,5 (1998): 561-5. doi: https://doi.org/10.1016/s0887-2333(98)00036-8
Imreh G, Beckman M, Iverfeldt K, Hallberg E. Development of Non-invasive Methods to Study Cell Death. Toxicol In Vitro. 1998 Oct;12(5):561-5. doi: 10.1016/s0887-2333(98)00036-8. PMID: 20654441.
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Toxicol In Vitro. 1998 Oct;12(5):561-5. doi: 10.1016/s0887-2333(98)00036-8.

Development of Non-invasive Methods to Study Cell Death.

Toxicology in vitro : an international journal published in association with BIBRA

G Imreh, M Beckman, K Iverfeldt, E Hallberg

Affiliations

  1. Department of Biochemistry, Stockholm, Sweden.

PMID: 20654441 DOI: 10.1016/s0887-2333(98)00036-8

Abstract

A novel non-invasive marker to monitor apoptosis in the living cell is presented. A human neuroblastoma cell line expressing an integral nuclear pore membrane protein tagged with GFP (green fluorescent protein) has been established, which enables monitoring of the nuclear envelope dynamics in intact cell cultures by fluorescence microscopy. During apoptosis, but not during necrosis, the GFP fluorescence around the nuclear rim disappears at a stage preceding nucleosomal DNA fragmentation. This phenomenon can thus be used as an early and convenient marker to specifically diagnose apoptotic development in cell cultures.

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