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Malgrange B, Lefebvre PP, van de Water TR, et al. In Vitro Ototoxicity of aminoglycosides and platin derivatives. A semi-automatic assay for sensory hair cell damage in explanted rat organ of corti. Toxicol In Vitro. 1998;12(6):705-14doi: 10.1016/s0887-2333(98)00048-4.
Malgrange, B., Lefebvre, P. P., van de Water, T. R., Bonnet, C., Monville, F., Rigo, J. M., Staecker, H., & Moonen, G. (1998). In Vitro Ototoxicity of aminoglycosides and platin derivatives. A semi-automatic assay for sensory hair cell damage in explanted rat organ of corti. Toxicology in vitro : an international journal published in association with BIBRA, 12(6), 705-14. https://doi.org/10.1016/s0887-2333(98)00048-4
Malgrange, B, et al. "In Vitro Ototoxicity of aminoglycosides and platin derivatives. A semi-automatic assay for sensory hair cell damage in explanted rat organ of corti." Toxicology in vitro : an international journal published in association with BIBRA vol. 12,6 (1998): 705-14. doi: https://doi.org/10.1016/s0887-2333(98)00048-4
Malgrange B, Lefebvre PP, van de Water TR, Bonnet C, Monville F, Rigo JM, Staecker H, Moonen G. In Vitro Ototoxicity of aminoglycosides and platin derivatives. A semi-automatic assay for sensory hair cell damage in explanted rat organ of corti. Toxicol In Vitro. 1998 Dec;12(6):705-14. doi: 10.1016/s0887-2333(98)00048-4. PMID: 20654460.
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Toxicol In Vitro. 1998 Dec;12(6):705-14. doi: 10.1016/s0887-2333(98)00048-4.

In Vitro Ototoxicity of aminoglycosides and platin derivatives. A semi-automatic assay for sensory hair cell damage in explanted rat organ of corti.

Toxicology in vitro : an international journal published in association with BIBRA

B Malgrange, P P Lefebvre, T R van de Water, C Bonnet, F Monville, J M Rigo, H Staecker, G Moonen

Affiliations

  1. Department of Human Physiology and Pathophysiology, University of Liège, 17 place delcour, B-4020, Liège, Belgium.

PMID: 20654460 DOI: 10.1016/s0887-2333(98)00048-4

Abstract

The ototoxic damage that drugs such as neomycin, kanamycin, colistin, cisplatin, transplatin and carboplatin cause on outer and inner hair cells in postnatal day 3 rat cochlear explants was investigated. Phalloidin-fluorescein conjugate-stained stereocilia bundles of sensory hair cells were quantified by video image analysis as a measurement of ototoxic effect. The video image quantification system established dose-response curves for ototoxic drugs (e.g. calculation of an IC(50)) and allowed comparisons between several ototoxins from the same family. This methodology provided the means to assess the efficacy of otoprotectant agents in preventing ototoxicity. Poly-l-aspartate (10(-5)m) and poly-l-glutamate (10(-5)m) protected auditory hair cells from neomycin (10(-3)m) toxicity while reduced glutathione (10(-3)m) provided protection against cisplatin (10(-4)m)-induced hair cell damage.

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