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Bondy GS, Armstrong CL, Dawson BA, et al. Toxicity of structurally related anthraquinones and anthrones to mammalian cells in vitro. Toxicol In Vitro. 1994;8(3):329-35doi: 10.1016/0887-2333(94)90153-8.
Bondy, G. S., Armstrong, C. L., Dawson, B. A., Héroux-Metcalf, C., Neville, G. A., & Rogers, C. G. (1994). Toxicity of structurally related anthraquinones and anthrones to mammalian cells in vitro. Toxicology in vitro : an international journal published in association with BIBRA, 8(3), 329-35. https://doi.org/10.1016/0887-2333(94)90153-8
Bondy, G S, et al. "Toxicity of structurally related anthraquinones and anthrones to mammalian cells in vitro." Toxicology in vitro : an international journal published in association with BIBRA vol. 8,3 (1994): 329-35. doi: https://doi.org/10.1016/0887-2333(94)90153-8
Bondy GS, Armstrong CL, Dawson BA, Héroux-Metcalf C, Neville GA, Rogers CG. Toxicity of structurally related anthraquinones and anthrones to mammalian cells in vitro. Toxicol In Vitro. 1994 Jun;8(3):329-35. doi: 10.1016/0887-2333(94)90153-8. PMID: 20692923.
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Toxicol In Vitro. 1994 Jun;8(3):329-35. doi: 10.1016/0887-2333(94)90153-8.

Toxicity of structurally related anthraquinones and anthrones to mammalian cells in vitro.

Toxicology in vitro : an international journal published in association with BIBRA

G S Bondy, C L Armstrong, B A Dawson, C Héroux-Metcalf, G A Neville, C G Rogers

Affiliations

  1. Toxicology Research Division, Bureau of Chemical Safety, Food Directorate, Health Protection Branch, Health and Welfare Canada, Ottawa, Ontario, Canada K1A 0L2.

PMID: 20692923 DOI: 10.1016/0887-2333(94)90153-8

Abstract

Anthraquinones and structurally related compounds were cytotoxic to mammalian cell lines using cloning efficiency and MTT reduction as endpoints. In V79 cells, the concentration of chemical causing 50% inhibition ranged from 0.21 to 21.6 mug/ml for cloning efficiency and from 0.86 to 14.6 mug/ml for MTT reduction. The anthrones anthralin and chrysarobin were 4.1 and 3.2 times more toxic, respectively, in the cloning efficiency assay than in the MTT assay. In contrast, the anthraquinones danthron and emodin were 2.8 and 2.1 times more toxic, respectively, in the MTT assay than in the cloning efficiency assay. Among the four mammalian cell lines tested using the MTT assay, the human leukaemia cell line (K562) was the most sensitive to the test chemicals. In contast, anthraquinone toxicity was reduced in rat hepatoma (H4IIE) cultures. In general, structures with carbonyl groups in positions 9 and 10 on the anthracene skeleton (anthraquinones) were less toxic than structures with carbonyl groups in position 9 only (anthrones). Toxicity was also influenced by the position of hydroxy substituents on the tricyclic skeleton. The results suggested that in vitro cytotoxicity assays are useful in elucidating the relationships between structure and biological activity for anthraquinones and related compounds.

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