J Appl Biomater Biomech. 2009 Sep-Dec;7(3):194-9.

Fabrication of chemically cross-linked porous gelatin matrices.

Journal of applied biomaterials & biomechanics : JABB

Sabrina Bozzini, Paola Petrini, Lina Altomare, Maria Cristina Tanzi

PMID: 20740429

Abstract

PURPOSE: The aim of this study was to chemically cross-link gelatin, by reacting its free amino groups with an aliphatic diisocyanate.

METHODS: To produce hydrogels with controllable properties, the number of reacting amino groups was carefully determined. Porosity was introduced into the gelatin-based hydrogels through the lyophilization process. Porous and non-porous matrices were characterized with respect to their chemical structure, morphology, water uptake and mechanical properties.

RESULTS: The physical, chemical and mechanical properties of the porous matrices are related to the extent of their cross-linking, showing that they can be controlled by varying the reaction parameters. Water uptake values (24 hours) vary between 160% and 200% as the degree of cross-linking increases. The flexibility of the samples also decreases by changing the extent of cross-linking. Young's modulus shows values between 0.188 KPa, for the highest degree, and 0.142 KPa for the lowest degree.

CONCLUSIONS: The matrices are potential candidates for use as tissue-engineering scaffolds by modulating their physical chemical properties according to the specific application.

Publication Types

• Journal Article