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Kleefen A, Pedone D, Grunwald C, et al. Multiplexed parallel single transport recordings on nanopore arrays. Nano Lett. 2010;10(12):5080-7doi: 10.1021/nl1033528.
Kleefen, A., Pedone, D., Grunwald, C., Wei, R., Firnkes, M., Abstreiter, G., Rant, U., & Tampé, R. (2010). Multiplexed parallel single transport recordings on nanopore arrays. Nano letters, 10(12), 5080-7. https://doi.org/10.1021/nl1033528
Kleefen, Alexander, et al. "Multiplexed parallel single transport recordings on nanopore arrays." Nano letters vol. 10,12 (2010): 5080-7. doi: https://doi.org/10.1021/nl1033528
Kleefen A, Pedone D, Grunwald C, Wei R, Firnkes M, Abstreiter G, Rant U, Tampé R. Multiplexed parallel single transport recordings on nanopore arrays. Nano Lett. 2010 Dec 08;10(12):5080-7. doi: 10.1021/nl1033528. Epub 2010 Oct 27. PMID: 20979410.
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Nano Lett. 2010 Dec 08;10(12):5080-7. doi: 10.1021/nl1033528. Epub 2010 Oct 27.

Multiplexed parallel single transport recordings on nanopore arrays.

Nano letters

Alexander Kleefen, Daniel Pedone, Christian Grunwald, Ruoshan Wei, Matthias Firnkes, Gerhard Abstreiter, Ulrich Rant, Robert Tampé

Affiliations

  1. Institute of Biochemistry, Biocenter, Center for Membrane Proteomics (CMP), and Cluster of Excellence Frankfurt (CEF)-Macromolecular Complexes, Goethe-University Frankfurt, Max-von-Laue-Strasse 9, D-60438 Frankfurt/M., Germany.

PMID: 20979410 DOI: 10.1021/nl1033528

Abstract

We introduce a nanofabricated silicon chip for massively multiplexed analysis of membrane channels and transporters in suspended lipid membranes that does not require any surface modification or organic solvent. Transport processes through single membrane complexes are monitored by fluorescence. The chip consists of an array of well-defined nanopores, addressing an individual pyramidal back-reflecting 30-fL compartment. The setup allows simultaneous analyses of ∼1,000 single transmembrane events in one field of view, observing translocation kinetics of transmembrane complexes.

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