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Watson BO, Nikolenko V, Araya R, et al. Two-photon microscopy with diffractive optical elements and spatial light modulators. Front Neurosci. 2010;4doi: 10.3389/fnins.2010.00029.
Watson, B. O., Nikolenko, V., Araya, R., Peterka, D. S., Woodruff, A., & Yuste, R. (2010). Two-photon microscopy with diffractive optical elements and spatial light modulators. Frontiers in neuroscience, 4. https://doi.org/10.3389/fnins.2010.00029
Watson, Brendon O, et al. "Two-photon microscopy with diffractive optical elements and spatial light modulators." Frontiers in neuroscience vol. 4 (2010). doi: https://doi.org/10.3389/fnins.2010.00029
Watson BO, Nikolenko V, Araya R, Peterka DS, Woodruff A, Yuste R. Two-photon microscopy with diffractive optical elements and spatial light modulators. Front Neurosci. 2010 Sep 15;4. doi: 10.3389/fnins.2010.00029. eCollection 2010. PMID: 20859526; PMCID: PMC2940544.
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Front Neurosci. 2010 Sep 15;4. doi: 10.3389/fnins.2010.00029. eCollection 2010.

Two-photon microscopy with diffractive optical elements and spatial light modulators.

Frontiers in neuroscience

Brendon O Watson, Volodymyr Nikolenko, Roberto Araya, Darcy S Peterka, Alan Woodruff, Rafael Yuste

Affiliations

  1. Howard Hughes Medical Institute, Department of Biological Sciences, Columbia University New York, NY, USA.

PMID: 20859526 PMCID: PMC2940544 DOI: 10.3389/fnins.2010.00029

Abstract

Two-photon microscopy is often performed at slow frame rates due to the need to serially scan all points in a field of view with a single laser beam. To overcome this problem, we have developed two optical methods that split and multiplex a laser beam across the sample. In the first method a diffractive optical element (DOE) generates a fixed number of beamlets that are scanned in parallel resulting in a corresponding increase in speed or in signal-to-noise ratio in time-lapse measurements. The second method uses a computer-controlled spatial light modulator (SLM) to generate any arbitrary spatio-temporal light pattern. With an SLM one can image or photostimulate any predefined region of the image such as neurons or dendritic spines. In addition, SLMs can be used to mimic a large number of optical transfer functions including light path corrections as adaptive optics.

Keywords: calcium imaging; diffractive optical element; imaging; spatial light modulator; two-photon microscopy

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