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Nakamura M, Kuramasu A, Nakashima I, et al. Candidate antigens specifically detected by cerebrospinal fluid-IgG in oligoclonal IgG bands-positive multiple sclerosis patients. Proteomics Clin Appl. 2007;1(7):681-7doi: 10.1002/prca.200700182.
Nakamura, M., Kuramasu, A., Nakashima, I., Fujihara, K., & Itoyama, Y. (2007). Candidate antigens specifically detected by cerebrospinal fluid-IgG in oligoclonal IgG bands-positive multiple sclerosis patients. Proteomics. Clinical applications, 1(7), 681-7. https://doi.org/10.1002/prca.200700182
Nakamura, Masashi, et al. "Candidate antigens specifically detected by cerebrospinal fluid-IgG in oligoclonal IgG bands-positive multiple sclerosis patients." Proteomics. Clinical applications vol. 1,7 (2007): 681-7. doi: https://doi.org/10.1002/prca.200700182
Nakamura M, Kuramasu A, Nakashima I, Fujihara K, Itoyama Y. Candidate antigens specifically detected by cerebrospinal fluid-IgG in oligoclonal IgG bands-positive multiple sclerosis patients. Proteomics Clin Appl. 2007 Jul;1(7):681-7. doi: 10.1002/prca.200700182. Epub 2007 Jun 08. PMID: 21136723.
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Proteomics Clin Appl. 2007 Jul;1(7):681-7. doi: 10.1002/prca.200700182. Epub 2007 Jun 08.

Candidate antigens specifically detected by cerebrospinal fluid-IgG in oligoclonal IgG bands-positive multiple sclerosis patients.

Proteomics. Clinical applications

Masashi Nakamura, Atsuo Kuramasu, Ichiro Nakashima, Kazuo Fujihara, Yasuto Itoyama

Affiliations

  1. Department of Neurology, Tohoku University School of Medicine, Aoba-ku, Sendai, Japan. [email protected].

PMID: 21136723 DOI: 10.1002/prca.200700182

Abstract

The aim of the present study was to detect antigenic proteins that react specifically with cerebrospinal fluid (CSF)-IgG from oligoclonal IgG bands (OB)-positive multiple sclerosis (MS) patients. To identify such antigenic proteins, we developed a rat brain proteome map using 2-DE and applied it to the immunoscreening of brain proteins that react with CSF-IgG but not with serum-IgG in OB-positive MS patients. After sequential MALDI-TOF mass spectrometry, eight proteins [two neuronal proteins (tubulin β-2 and γ enolase-2), HSP-1, Tpi-1 protein and cellular enzymes (creatine kinase, phosphopyruvate hydratase, triosephosphate isomerase and phosphoglycerate kinase-1)] were identified as candidate antigens in seven MS patients. Reactivity to tubulin was seen in Western blotting in four patients, and CSF-specific anti-tubulin IgG was detected in one patient. In addition, CSF-specific anti-gamma enolase IgG was found in another patient. These findings suggest that intrathecal immune responses may occur against a broad range of proteins in MS.

Copyright © 2007 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

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