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Lescuyer P, Pernin A, Hainard A, et al. Proteomic analysis of a podocyte vesicle-enriched fraction from human normal and pathological urine samples. Proteomics Clin Appl. 2008;2(7):1008-18doi: 10.1002/prca.200800033.
Lescuyer, P., Pernin, A., Hainard, A., Bigeire, C., Burgess, J. A., Zimmermann-Ivol, C., Sanchez, J. C., Schifferli, J. A., Hochstrasser, D. F., & Moll, S. (2008). Proteomic analysis of a podocyte vesicle-enriched fraction from human normal and pathological urine samples. Proteomics. Clinical applications, 2(7), 1008-18. https://doi.org/10.1002/prca.200800033
Lescuyer, Pierre, et al. "Proteomic analysis of a podocyte vesicle-enriched fraction from human normal and pathological urine samples." Proteomics. Clinical applications vol. 2,7 (2008): 1008-18. doi: https://doi.org/10.1002/prca.200800033
Lescuyer P, Pernin A, Hainard A, Bigeire C, Burgess JA, Zimmermann-Ivol C, Sanchez JC, Schifferli JA, Hochstrasser DF, Moll S. Proteomic analysis of a podocyte vesicle-enriched fraction from human normal and pathological urine samples. Proteomics Clin Appl. 2008 Jul;2(7):1008-18. doi: 10.1002/prca.200800033. Epub 2008 Jul 10. PMID: 21136901.
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Proteomics Clin Appl. 2008 Jul;2(7):1008-18. doi: 10.1002/prca.200800033. Epub 2008 Jul 10.

Proteomic analysis of a podocyte vesicle-enriched fraction from human normal and pathological urine samples.

Proteomics. Clinical applications

Pierre Lescuyer, Agnès Pernin, Alexandre Hainard, Caty Bigeire, Jennifer A Burgess, Catherine Zimmermann-Ivol, Jean-Charles Sanchez, Jürg A Schifferli, Denis F Hochstrasser, Solange Moll

Affiliations

  1. Biomedical Proteomics Research Group, University Hospital, Geneva, Switzerland.

PMID: 21136901 DOI: 10.1002/prca.200800033

Abstract

Podocytes (glomerular visceral epithelial cells) release vesicles into urine. Podocyte vesicle-enriched fractions from normal and pathological human urine samples were prepared for proteomic analysis. An immunoadsorption method was applied and enrichment of podocyte vesicles was assessed. We identified 76 unique proteins. One protein, serum paraoxonase/arylesterase 1 (PON-1), was newly identified in normal human urine sample. We confirmed this result and showed PON-1 expression in normal human kidney. These results demonstrated the potential for using the urine samples enriched in podocyte vesicles as a starting material in studies aimed at discovery of biomarkers for diseases.

Copyright © 2008 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

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