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Sexton JZ, Ackerson CJ. Determination of Rigidity of Protein Bound Au(144) Clusters by Electron Cryomicroscopy. J Phys Chem C Nanomater Interfaces. 2010;114(38):16037-16042doi: 10.1021/jp101970x.
Sexton, J. Z., & Ackerson, C. J. (2010). Determination of Rigidity of Protein Bound Au(144) Clusters by Electron Cryomicroscopy. The journal of physical chemistry. C, Nanomaterials and interfaces, 114(38), 16037-16042. https://doi.org/10.1021/jp101970x
Sexton, Jonathan Z, and Ackerson, Christopher J. "Determination of Rigidity of Protein Bound Au(144) Clusters by Electron Cryomicroscopy." The journal of physical chemistry. C, Nanomaterials and interfaces vol. 114,38 (2010): 16037-16042. doi: https://doi.org/10.1021/jp101970x
Sexton JZ, Ackerson CJ. Determination of Rigidity of Protein Bound Au(144) Clusters by Electron Cryomicroscopy. J Phys Chem C Nanomater Interfaces. 2010 Sep 30;114(38):16037-16042. doi: 10.1021/jp101970x. PMID: 21116473; PMCID: PMC2992337.
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J Phys Chem C Nanomater Interfaces. 2010 Sep 30;114(38):16037-16042. doi: 10.1021/jp101970x.

Determination of Rigidity of Protein Bound Au(144) Clusters by Electron Cryomicroscopy.

The journal of physical chemistry. C, Nanomaterials and interfaces

Jonathan Z Sexton, Christopher J Ackerson

Affiliations

  1. Department of Structural Biology, 299 Campus Drive West, Stanford, CA 94305.

PMID: 21116473 PMCID: PMC2992337 DOI: 10.1021/jp101970x

Abstract

A method for estimating the positional displacement of protein bound gold nanoparticles is presented and used to estimate the rigidity of linkage of Au(144) nanoparticles bound to a tetrameric model protein. We observe a distribution of displacement values where most Au(144) clusters are immobilized to within 3Å relative to the protein center of mass. The shape of the distribution suggests two physical processes of thermal motion and protein deformation. The application of this and similar rigid gold nanoparticle/protein conjugates in high resolution single particle electron cryo-microscopy is discussed.

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