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Clay CM, Nelson SE, Digregorio GB, et al. Cell-specific expression of the mouse gonadotropin-releasing hormone (GnRH) receptor gene is conferred by elements residing within 500 bp of proximal 5' flanking region. Endocrine. 1995;3(8):615-22doi: 10.1007/BF02953028.
Clay, C. M., Nelson, S. E., Digregorio, G. B., Campion, C. E., Wiedemann, A. L., & Nett, R. J. (1995). Cell-specific expression of the mouse gonadotropin-releasing hormone (GnRH) receptor gene is conferred by elements residing within 500 bp of proximal 5' flanking region. Endocrine, 3(8), 615-22. https://doi.org/10.1007/BF02953028
Clay, C M, et al. "Cell-specific expression of the mouse gonadotropin-releasing hormone (GnRH) receptor gene is conferred by elements residing within 500 bp of proximal 5' flanking region." Endocrine vol. 3,8 (1995): 615-22. doi: https://doi.org/10.1007/BF02953028
Clay CM, Nelson SE, Digregorio GB, Campion CE, Wiedemann AL, Nett RJ. Cell-specific expression of the mouse gonadotropin-releasing hormone (GnRH) receptor gene is conferred by elements residing within 500 bp of proximal 5' flanking region. Endocrine. 1995 Aug;3(8):615-22. doi: 10.1007/BF02953028. PMID: 21153141.
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Endocrine. 1995 Aug;3(8):615-22. doi: 10.1007/BF02953028.

Cell-specific expression of the mouse gonadotropin-releasing hormone (GnRH) receptor gene is conferred by elements residing within 500 bp of proximal 5' flanking region.

Endocrine

C M Clay, S E Nelson, G B Digregorio, C E Campion, A L Wiedemann, R J Nett

Affiliations

  1. Animal Reproduction and Biotechnology Laboratory, Department of Physiology, Colorado State University, Fort Collins, 80523, Colorado, USA.

PMID: 21153141 DOI: 10.1007/BF02953028

Abstract

Gonadotropin-releasing hormone (GnRH) is a decapeptide produced by the hypothalamus. Upon binding to specific high-affinity receptors on gonadotrope cells of the anterior pituitary gland, GnRH stimulates the synthesis and secretion of LH. In light of the critical role of GnRH in reproduction much effort has been directed toward understanding the regulation of this hormone and its cognate receptor. The recent availability of genomic clones for the GnRH receptor has facilitated research to address the molecular mechanisms underlying regulation of GnRH receptor gene expression. We have expanded the analysis of the promoter for the mouse GnRH receptor gene and report that in addition to transcriptional start sites located within 100 bp of the translation start codon there is a more distal transcriptional start site approximately 200 bp 5' of the initiation codon. The initiation of transcription from this more distal site was sufficient to confer cell-specific expression on luciferase. Further, transient expression assays of constructs containing progressive 5' deletions in the GnRH receptor gene promoter reveal the presence of one or morecis-acting elements located between -500 and -400 (relative to ATG) necessary for transcriptional activity in the gonadotrope-derived αT3 cell line. Finally, αT3 but not COS-7 cell nuclear extract contained protein(s) that bind to at least two separate motifs contained within the -500 to -400 region. We suggest that activation of GnRH receptor gene expression in the αT3 cell line requires the binding of at least two transcriptional regulatory proteins to basal enhancer elements located within a 100 bp region between -500 to -400 relative to the translation start codon in the mouse GnRH receptor gene.

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