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Halloran MM, Emanuele MA, Emanuele NV, et al. Further characterization of the impact of ethanol on βLH: alterations in polyribosome association of βLH mRNA. Endocrine. 1995;3(6):469-73doi: 10.1007/BF02935654.
Halloran, M. M., Emanuele, M. A., Emanuele, N. V., Tentler, J. J., & Kelley, M. R. (1995). Further characterization of the impact of ethanol on βLH: alterations in polyribosome association of βLH mRNA. Endocrine, 3(6), 469-73. https://doi.org/10.1007/BF02935654
Halloran, M M, et al. "Further characterization of the impact of ethanol on βLH: alterations in polyribosome association of βLH mRNA." Endocrine vol. 3,6 (1995): 469-73. doi: https://doi.org/10.1007/BF02935654
Halloran MM, Emanuele MA, Emanuele NV, Tentler JJ, Kelley MR. Further characterization of the impact of ethanol on βLH: alterations in polyribosome association of βLH mRNA. Endocrine. 1995 Jun;3(6):469-73. doi: 10.1007/BF02935654. PMID: 21153252.
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Endocrine. 1995 Jun;3(6):469-73. doi: 10.1007/BF02935654.

Further characterization of the impact of ethanol on βLH: alterations in polyribosome association of βLH mRNA.

Endocrine

M M Halloran, M A Emanuele, N V Emanuele, J J Tentler, M R Kelley

Affiliations

  1. Department of Molecular and Cellular Biochemistry, Loyola University of Chicago, Maywood, Illinois, USA.

PMID: 21153252 DOI: 10.1007/BF02935654

Abstract

We have previously reported a decrease in Luteinizing Hormone (LH) levels in serum afterin vivo acute ethanol exposure in male rats. Accompanying these changes, a rapid and marked decrease of β-LH mRNA was observed. A similar decrease was not detected in the common α-subunit or β-FSH mRNA. The studies presented here examined the possible mechanisms of decreasing β-LH mRNA by using S1 nuclease protection assay to evaluate the effect of acute ethanol exposure on the levels of β-LH heteronuclear RNA (hnRNA). There was no significant difference detected in the level of β-LH hnRNA after ethanol exposure. Polysome distribution analysis was used to evaluate the association and disassociation of β-LH mRNA with polyribosomes since non-polyribosome associated mRNA may be more vulnerable to degradation by RNAases. The results indicated a decrease in the association of the β-LH mRNA with polysomes following acute ethanol exposure. This decrease in polyribosome association would increase the exposure of the β-LH transcript making it more susceptible to RNases. We conclude that the decrease in steady-state β-LH mRNA levels after ethanol exposure occurs because of increasing degradation of the transcript rendered vulnerable by displacement from polysomes and not through a decreased transcriptional rate.

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