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Abdelwahab SI, Mohan S, Mohamed Elhassan M, et al. Antiapoptotic and Antioxidant Properties of Orthosiphon stamineus Benth (Cat's Whiskers): Intervention in the Bcl-2-Mediated Apoptotic Pathway. Evid Based Complement Alternat Med. 2010;2011:156765doi: 10.1155/2011/156765.
Abdelwahab, S. I., Mohan, S., Mohamed Elhassan, M., Al-Mekhlafi, N., Mariod, A. A., Abdul, A. B., Abdulla, M. A., & Alkharfy, K. M. (2011). Antiapoptotic and Antioxidant Properties of Orthosiphon stamineus Benth (Cat's Whiskers): Intervention in the Bcl-2-Mediated Apoptotic Pathway. Evidence-based complementary and alternative medicine : eCAM, 2011156765. https://doi.org/10.1155/2011/156765
Abdelwahab, Siddig Ibrahim, et al. "Antiapoptotic and Antioxidant Properties of Orthosiphon stamineus Benth (Cat's Whiskers): Intervention in the Bcl-2-Mediated Apoptotic Pathway." Evidence-based complementary and alternative medicine : eCAM vol. 2011 (2011): 156765. doi: https://doi.org/10.1155/2011/156765
Abdelwahab SI, Mohan S, Mohamed Elhassan M, Al-Mekhlafi N, Mariod AA, Abdul AB, Abdulla MA, Alkharfy KM. Antiapoptotic and Antioxidant Properties of Orthosiphon stamineus Benth (Cat's Whiskers): Intervention in the Bcl-2-Mediated Apoptotic Pathway. Evid Based Complement Alternat Med. 2011;2011:156765. doi: 10.1155/2011/156765. Epub 2010 Dec 30. PMID: 21234328; PMCID: PMC3018659.
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Evid Based Complement Alternat Med. 2011;2011:156765. doi: 10.1155/2011/156765. Epub 2010 Dec 30.

Antiapoptotic and Antioxidant Properties of Orthosiphon stamineus Benth (Cat's Whiskers): Intervention in the Bcl-2-Mediated Apoptotic Pathway.

Evidence-based complementary and alternative medicine : eCAM

Siddig Ibrahim Abdelwahab, Syam Mohan, Manal Mohamed Elhassan, Nabil Al-Mekhlafi, Abdelbasit Adam Mariod, Ahmad Bustamam Abdul, Mahmood Ameen Abdulla, Khalid M Alkharfy

Affiliations

  1. Department of Pharmacy, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.

PMID: 21234328 PMCID: PMC3018659 DOI: 10.1155/2011/156765

Abstract

Antiapoptotic and antioxidant activities of aqueous-methanolic extract (CAME) of Orthosiphonstamineus Benth(OS), and its hexane (HF), chloroform (CF), n-butanol (NBF), ethyl acetate (EAF) and water (WF) fractions were investigated. Antioxidant properties were evaluated using the assays of Folin-Ciocalteu, aluminiumtrichloride, β-carotene bleaching and DPPH. The role of OS against hydrogen peroxide induced apoptosis on MDA-M231 epithelial cells was examined using MTT assay, phase contrast microscope, colorimetric assay of caspase-3, western blot and quantitative real-time PCR. Results showed that EAF showed the highest total phenolic content followed by CAME, NBF, WF, CF and HF, respectively. Flavonoid content was in the order of the CF > EAF > HF > CAME > NBF > WF. The IC(50) values on DPPH assay for different extract/fractions were 126.2 ± 23, 31.25 ± 1.2, 15.25 ± 2.3, 13.56 ± 1.9, 23.0 ± 3.2, and 16.66 ± 1.5 μg/ml for HF, CF, EAF, NBF, WF and CAME, respectively. OSreduced the oxidation of β-carotene by hydroperoxides. Cell death was dose-dependently inhibited by pretreatment with OS. Caspase-3 and distinct morphological features suggest the anti-apoptotic activities of OS. This plant not only increased the expression of Bcl-2, but also decreased Bax expression, and ultimately reduced H(2)O(2)-induced apoptosis. The current results showed that phenolics may provide health and nutritional benefits.

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