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Hossain A, Aryal S, Osuampke C, et al. Human sperm bioassay for reprotoxicity testing in embryo culture media: some practical considerations in reducing the assay time. Adv Urol. 2010;2010:136898doi: 10.1155/2010/136898.
Hossain, A., Aryal, S., Osuampke, C., & Phelps, J. (2010). Human sperm bioassay for reprotoxicity testing in embryo culture media: some practical considerations in reducing the assay time. Advances in urology, 2010136898. https://doi.org/10.1155/2010/136898
Hossain, Amjad, et al. "Human sperm bioassay for reprotoxicity testing in embryo culture media: some practical considerations in reducing the assay time." Advances in urology vol. 2010 (2010): 136898. doi: https://doi.org/10.1155/2010/136898
Hossain A, Aryal S, Osuampke C, Phelps J. Human sperm bioassay for reprotoxicity testing in embryo culture media: some practical considerations in reducing the assay time. Adv Urol. 2010;2010:136898. doi: 10.1155/2010/136898. Epub 2010 Dec 19. PMID: 21234335; PMCID: PMC3017937.
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Adv Urol. 2010;2010:136898. doi: 10.1155/2010/136898. Epub 2010 Dec 19.

Human sperm bioassay for reprotoxicity testing in embryo culture media: some practical considerations in reducing the assay time.

Advances in urology

Amjad Hossain, Subhash Aryal, Collin Osuampke, John Phelps

Affiliations

  1. Division of Reproductive Endocrinology, Department of Obstetrics and Gynecology, The University of Texas Medical Branch, 301 University Boulevard, Galveston, TX 77555, USA.

PMID: 21234335 PMCID: PMC3017937 DOI: 10.1155/2010/136898

Abstract

Human sperm assay (HSA) is a preferred in house quality control and proficiency test (PT) practiced in fertility laboratories. HSA is performed over varying durations, apparently without following set criteria. To better understand the assay time required for reprotoxicity testing in embryo culture media, we compared American-Association-of-Bioanalysts-(AAB-) administered HSA data to our own assay performed using PT samples obtained from AAB. Participating laboratories were required to culture sperm for 48 hours to determine media acceptability. Conclusions drawn from 48- and 24-hour observations were the same, suggesting that HSA could identify reprotoxic media in less time than required by AAB. Our assay revealed that changes in motility grade in adulterated media are significantly different from those in control media. Furthermore, grade changes can be identified earlier than differences in motility loss between samples. Analyzing motility and motility quality together provides a method for establishing an optimal time for HSA.

References

  1. J In Vitro Fert Embryo Transf. 1988 Dec;5(6):335-42 - PubMed
  2. Semin Reprod Med. 2005 Nov;23(4):319-24 - PubMed
  3. Hum Reprod. 2000 Jul;15(7):1586-91 - PubMed
  4. J Androl. 2003 Jan-Feb;24(1):16-8 - PubMed
  5. Br J Clin Pharmacol. 1981 Apr;11(4):385-7 - PubMed
  6. Fertil Steril. 2009 Aug;92(2):527-35 - PubMed
  7. Fertil Steril. 1988 Mar;49(3):516-21 - PubMed
  8. J Reprod Fertil. 1974 Jun;38(2):431-40 - PubMed
  9. Hum Reprod. 1993 Jun;8(6):916-8 - PubMed
  10. Fertil Steril. 1996 Feb;65(2):400-5 - PubMed
  11. Hum Reprod. 1989 Jul;4(5):545-9 - PubMed
  12. Fertil Steril. 1993 Jan;59(1):192-6 - PubMed
  13. Fertil Steril. 2008 Jan;89(1):237-9 - PubMed
  14. J In Vitro Fert Embryo Transf. 1988 Apr;5(2):67-75 - PubMed
  15. Hum Reprod. 1997 Aug;12(8):1673-81 - PubMed
  16. Br J Obstet Gynaecol. 1980 Sep;87(9):737-56 - PubMed
  17. J Assist Reprod Genet. 2010 Feb;27(2-3):83-6 - PubMed
  18. Fertil Steril. 1995 Mar;63(3):660-2 - PubMed
  19. Arch Androl. 1996 Jul-Aug;37(1):7-10 - PubMed
  20. Hum Cell. 1997 Dec;10(4):271-6 - PubMed

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