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Kantevari S, Makara JK, Losonczy A, et al. Development of anionically decorated 2-(ortho-nitrophenyl)-propyl-caged neurotransmitters for photolysis in vitro and in vivo. Chembiochem. 2011;doi: 10.1002/cbic.201000254.
Kantevari, S., Makara, J. K., Losonczy, A., Fellin, T., Haydon, P. G., Magee, J. C., & Ellis-Davies, G. C. (2011). Development of anionically decorated 2-(ortho-nitrophenyl)-propyl-caged neurotransmitters for photolysis in vitro and in vivo. Chembiochem : a European journal of chemical biology, . https://doi.org/10.1002/cbic.201000254
Kantevari, Srinivas, et al. "Development of anionically decorated 2-(ortho-nitrophenyl)-propyl-caged neurotransmitters for photolysis in vitro and in vivo." Chembiochem : a European journal of chemical biology vol. (2011). doi: https://doi.org/10.1002/cbic.201000254
Kantevari S, Makara JK, Losonczy A, Fellin T, Haydon PG, Magee JC, Ellis-Davies GC. Development of anionically decorated 2-(ortho-nitrophenyl)-propyl-caged neurotransmitters for photolysis in vitro and in vivo. Chembiochem. 2011 Jan 10; doi: 10.1002/cbic.201000254. Epub 2011 Jan 10. PMID: 21225599.
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Chembiochem. 2011 Jan 10; doi: 10.1002/cbic.201000254. Epub 2011 Jan 10.

Development of anionically decorated 2-(ortho-nitrophenyl)-propyl-caged neurotransmitters for photolysis in vitro and in vivo.

Chembiochem : a European journal of chemical biology

Srinivas Kantevari, Judit K Makara, Attila Losonczy, Tommaso Fellin, Philip G Haydon, Jeffrey C Magee, Graham C R Ellis-Davies

Affiliations

  1. Department of Neuroscience, Mount Sinai School of Medicine, One Gustave Levy Place, New York, NY 10029 (USA).

PMID: 21225599 DOI: 10.1002/cbic.201000254

Abstract

Three new caged neurotransmitters were synthesized built around the 2-(ortho-nitrophenyl)propyl (NPP) caging chromophore. The NPP-caged L-glutamate (Glu) and γ-aminobutyric acid (GABA) derivatives, which have an extended π-electron system bearing two carboxylates or phosphates were highly soluble (>50 mM) and hydrolytically stable at physiological pH. Uncaging GABA with ultraviolet light blocked network oscillations in layer 1 of the neocortex of a living mouse. Two-photon photolysis of caged Glu at single spine heads evoked changes in membrane voltage that were identical to synaptic stimulations. The implications of solubility complexities for the further development of the NPP scaffold for neurotransmitter uncaging are discussed in the context of other recent developments in this area.

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