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Urbanek DC, Vorobyev DY, Serrano AL, et al. The Two Dimensional Vibrational Echo of a Nitrile Probe of the Villin HP35 Protein. J Phys Chem Lett. 2010;1:3311-3315doi: 10.1021/jz101367d.
Urbanek, D. C., Vorobyev, D. Y., Serrano, A. L., Gai, F., & Hochstrasser, R. M. (2010). The Two Dimensional Vibrational Echo of a Nitrile Probe of the Villin HP35 Protein. The journal of physical chemistry letters, 13311-3315. https://doi.org/10.1021/jz101367d
Urbanek, Diana C, et al. "The Two Dimensional Vibrational Echo of a Nitrile Probe of the Villin HP35 Protein." The journal of physical chemistry letters vol. 1 (2010): 3311-3315. doi: https://doi.org/10.1021/jz101367d
Urbanek DC, Vorobyev DY, Serrano AL, Gai F, Hochstrasser RM. The Two Dimensional Vibrational Echo of a Nitrile Probe of the Villin HP35 Protein. J Phys Chem Lett. 2010;1:3311-3315. doi: 10.1021/jz101367d. PMID: 21132120; PMCID: PMC2995499.
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J Phys Chem Lett. 2010;1:3311-3315. doi: 10.1021/jz101367d.

The Two Dimensional Vibrational Echo of a Nitrile Probe of the Villin HP35 Protein.

The journal of physical chemistry letters

Diana C Urbanek, Dmitriy Yu Vorobyev, Arnaldo L Serrano, Feng Gai, Robin M Hochstrasser

Affiliations

  1. Department of Chemistry, University of Pennsylvania, Philadelphia, Pennsylvania 19104-6323.

PMID: 21132120 PMCID: PMC2995499 DOI: 10.1021/jz101367d

Abstract

2D IR spectroscopy was used to probe the hydrophobic core structure of the 35-residue Villin headpiece subdomain, HP35, by monitoring the C≡N vibrational stretching band of a cyano substituted phenylalanine (Phe). The presence of two humps in the vibrational frequency distribution in the folded equilibrium state is revealed. They represent two states that exchange more slowly than ca. 10 ps. The two CN stretch mode peak frequencies (and their equilibrium populations) are 2228.7 (44%) and 2234.5 cm(-1) (56%). The two CN modes have different frequency-frequency correlation times of 7.4 ps and 1.6 ps respectively. These results suggest that the population with the higher frequency CN group is partly exposed whereas the other CN mode experiences a hydrophobic like environment.

References

  1. Proc Natl Acad Sci U S A. 2010 Jun 15;107(24):10890-5 - PubMed
  2. J Phys Chem B. 2010 Oct 28;114(42):13536-44 - PubMed
  3. Proc Natl Acad Sci U S A. 2008 Dec 2;105(48):18655-62 - PubMed
  4. Curr Opin Struct Biol. 2009 Apr;19(2):120-7 - PubMed
  5. J Chem Phys. 2008 Apr 21;128(15):154504 - PubMed
  6. J Am Chem Soc. 2004 Apr 28;126(16):5078-9 - PubMed
  7. Biophys J. 2009 Oct 21;97(8):2338-47 - PubMed
  8. Proc Natl Acad Sci U S A. 2010 May 18;107(20):9152-7 - PubMed
  9. Proc Natl Acad Sci U S A. 2005 Aug 9;102(32):11185-90 - PubMed
  10. Proc Natl Acad Sci U S A. 2005 Nov 15;102(46):16662-7 - PubMed
  11. Biophys J. 2009 May 20;96(10):4176-87 - PubMed
  12. Proc Natl Acad Sci U S A. 2005 May 24;102(21):7517-22 - PubMed
  13. Chem Rev. 2010 Mar 10;110(3):1498-517 - PubMed
  14. J Chem Phys. 2009 May 28;130(20):204509 - PubMed
  15. J Am Chem Soc. 2003 Jan 15;125(2):405-11 - PubMed
  16. Proc Natl Acad Sci U S A. 2008 Feb 5;105(5):1472-7 - PubMed
  17. Chem Phys Lett. 2009 Feb 17;469(4-6):325-330 - PubMed
  18. Protein Sci. 2002 Mar;11(3):680-7 - PubMed
  19. Nat Struct Biol. 1997 Mar;4(3):180-4 - PubMed
  20. Proc Natl Acad Sci U S A. 2007 Sep 4;104(36):14190-6 - PubMed
  21. Proc Natl Acad Sci U S A. 2010 Mar 16;107(11):4955-60 - PubMed
  22. Biochemistry. 2007 Oct 30;46(43):12308-13 - PubMed
  23. J Phys Chem B. 2008 May 22;112(20):6301-3 - PubMed
  24. Angew Chem Int Ed Engl. 2009;48(21):3750-81 - PubMed
  25. J Phys Chem B. 2005 Jun 2;109(21):11016-26 - PubMed
  26. Proc Natl Acad Sci U S A. 2007 Sep 4;104(36):14209-14 - PubMed
  27. Proc Natl Acad Sci U S A. 2000 Jul 18;97(15):8219-24 - PubMed
  28. Biochemistry. 2009 Jun 2;48(21):4607-16 - PubMed
  29. J Am Chem Soc. 2009 Jun 3;131(21):7470-6 - PubMed
  30. J Phys Chem B. 2009 Jun 18;113(24):8231-51 - PubMed
  31. J Phys Chem B. 2005 Apr 21;109(15):7511-21 - PubMed

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