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Moser R, Chappuis T, Vanoli E, et al. Continuous micro-production using enzymatic reaction and online monitoring. Chimia (Aarau). 2010;64(11):799-802doi: 10.2533/chimia.2010.799.
Moser, R., Chappuis, T., Vanoli, E., Crelier, S., & Naef, O. (2010). Continuous micro-production using enzymatic reaction and online monitoring. Chimia, 64(11), 799-802. https://doi.org/10.2533/chimia.2010.799
Moser, Regula, et al. "Continuous micro-production using enzymatic reaction and online monitoring." Chimia vol. 64,11 (2010): 799-802. doi: https://doi.org/10.2533/chimia.2010.799
Moser R, Chappuis T, Vanoli E, Crelier S, Naef O. Continuous micro-production using enzymatic reaction and online monitoring. Chimia (Aarau). 2010;64(11):799-802. doi: 10.2533/chimia.2010.799. PMID: 21197844.
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Chimia (Aarau). 2010;64(11):799-802. doi: 10.2533/chimia.2010.799.

Continuous micro-production using enzymatic reaction and online monitoring.

Chimia

Regula Moser, Thierry Chappuis, Ennio Vanoli, Simon Crelier, Olivier Naef

Affiliations

  1. Ecole d'ingénieurs et d'architectes de Fribourg Boulevard de Pérolles 80.

PMID: 21197844 DOI: 10.2533/chimia.2010.799

Abstract

A micro-reactor coupled to a microfluidic system and an online UV/VIS spectrometer is described. The enzymatic reaction studied is the hydrolysis of the N-benzoyl-L-tyrosine ethyl ester (BTEE) to N-benzoyl-L-tyrosine (BT) and ethanol, catalyzed by chymotrypsin. The production is online monitored with UV spectroscopy at 256 nm. Three different immobilization methods of the enzyme are discussed: Eupergit C, controlled-pore glass (CPG), and Sepharose.

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