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Emam A, Carter WG, Lingwood C. Glycolipid-Dependent, Protease Sensitive Internalization of Pseudomonas aeruginosa Into Cultured Human Respiratory Epithelial Cells. Open Microbiol J. 2010;4:106-15doi: 10.2174/1874285801004010106.
Emam, A., Carter, W. G., & Lingwood, C. (2010). Glycolipid-Dependent, Protease Sensitive Internalization of Pseudomonas aeruginosa Into Cultured Human Respiratory Epithelial Cells. The open microbiology journal, 4106-15. https://doi.org/10.2174/1874285801004010106
Emam, Aufaugh, et al. "Glycolipid-Dependent, Protease Sensitive Internalization of Pseudomonas aeruginosa Into Cultured Human Respiratory Epithelial Cells." The open microbiology journal vol. 4 (2010): 106-15. doi: https://doi.org/10.2174/1874285801004010106
Emam A, Carter WG, Lingwood C. Glycolipid-Dependent, Protease Sensitive Internalization of Pseudomonas aeruginosa Into Cultured Human Respiratory Epithelial Cells. Open Microbiol J. 2010 Dec 13;4:106-15. doi: 10.2174/1874285801004010106. PMID: 21270937; PMCID: PMC3026333.
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Open Microbiol J. 2010 Dec 13;4:106-15. doi: 10.2174/1874285801004010106.

Glycolipid-Dependent, Protease Sensitive Internalization of Pseudomonas aeruginosa Into Cultured Human Respiratory Epithelial Cells.

The open microbiology journal

Aufaugh Emam, William G Carter, Clifford Lingwood

Affiliations

  1. Molecular Structure and Function, The Research Institute, Hospital for Sick Children, Toronto, Ontario M5G 1X8, Canada.

PMID: 21270937 PMCID: PMC3026333 DOI: 10.2174/1874285801004010106

Abstract

Internalization of PAK strain Pseudomonas aeruginosa into human respiratory epithelial cell lines and HeLa cervical cancer cells in vitro was readily demonstrable via a gentamycin protection assay. Depletion of target cell glycosphingolipids (GSLs) using a glucosyl ceramide synthase inhibitor, P4, completely prevented P. aeruginosa internalization. In contrast, P4 treatment had no effect on the internalization of Salmonella typhimurium into HeLa cells. Internalized P. aeruginosa were within membrane vacuoles, often containing microvesicles, between the bacterium and the limiting membrane. P. aeruginosa internalization was markedly enhanced by target cell pretreatment with the exogenous GSL, deacetyl gangliotetraosyl ceramide (Gg(4)). Gg(4) binds the lipid raft marker, GM1 ganglioside. Target cell pretreatment with TLCK, but not other (serine) protease inhibitors, prevented both P. aeruginosa host cell binding and internalization. NFkB inhibition also prevented internalization. A GSL-containing lipid-raft model of P. aeruginosa host cell binding/internalization is proposed.

Keywords: Src kinase; carbohydrate/carbohydrate binding; gangliotetraosyl ceramide; gp140.; lipid rafts

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