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Smith MB, McGillivray DJ, Genzer J, et al. Neutron reflectometry of supported hybrid bilayers with inserted peptide. Soft Matter. 2010;6(5):862-865doi: 10.1039/b915800f.
Smith, M. B., McGillivray, D. J., Genzer, J., Lösche, M., & Kilpatrick, P. K. (2010). Neutron reflectometry of supported hybrid bilayers with inserted peptide. Soft matter, 6(5), 862-865. https://doi.org/10.1039/b915800f
Smith, Matthew B, et al. "Neutron reflectometry of supported hybrid bilayers with inserted peptide." Soft matter vol. 6,5 (2010): 862-865. doi: https://doi.org/10.1039/b915800f
Smith MB, McGillivray DJ, Genzer J, Lösche M, Kilpatrick PK. Neutron reflectometry of supported hybrid bilayers with inserted peptide. Soft Matter. 2010;6(5):862-865. doi: 10.1039/b915800f. PMID: 21274414; PMCID: PMC3026316.
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Soft Matter. 2010;6(5):862-865. doi: 10.1039/b915800f.

Neutron reflectometry of supported hybrid bilayers with inserted peptide.

Soft matter

Matthew B Smith, Duncan J McGillivray, Jan Genzer, Mathias Lösche, Peter K Kilpatrick

Affiliations

  1. Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, North Carolina, USA.

PMID: 21274414 PMCID: PMC3026316 DOI: 10.1039/b915800f

Abstract

The insertion of a synthetic amphiphilic, α-helical peptide into a supported hybrid bilayer membrane (HBM) was studied by neutron reflectometry to elucidate the resulting nanostructure. The HBM consisted of a self-assembled monolayer of perdeuterated octadecanethiol on gold and an overlying leaflet of acyl-deuterated phosphatidylcholine (d-DMPC). Using contrast variation, several reflectivity spectra were recorded for each step of film fabrication, and simultaneously modeled. This analysis indicated that peptide insertion into the DMPC lipid leaflet is the likeliest mode of incorporation.

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