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Toh DF, Patel DN, Chan EC, et al. Anti-proliferative effects of raw and steamed extracts of Panax notoginseng and its ginsenoside constituents on human liver cancer cells. Chin Med. 2011;6:4doi: 10.1186/1749-8546-6-4.
Toh, D. F., Patel, D. N., Chan, E. C., Teo, A., Neo, S. Y., & Koh, H. L. (2011). Anti-proliferative effects of raw and steamed extracts of Panax notoginseng and its ginsenoside constituents on human liver cancer cells. Chinese medicine, 64. https://doi.org/10.1186/1749-8546-6-4
Toh, Ding-Fung, et al. "Anti-proliferative effects of raw and steamed extracts of Panax notoginseng and its ginsenoside constituents on human liver cancer cells." Chinese medicine vol. 6 (2011): 4. doi: https://doi.org/10.1186/1749-8546-6-4
Toh DF, Patel DN, Chan EC, Teo A, Neo SY, Koh HL. Anti-proliferative effects of raw and steamed extracts of Panax notoginseng and its ginsenoside constituents on human liver cancer cells. Chin Med. 2011 Jan 24;6:4. doi: 10.1186/1749-8546-6-4. PMID: 21255464; PMCID: PMC3037355.
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Chin Med. 2011 Jan 24;6:4. doi: 10.1186/1749-8546-6-4.

Anti-proliferative effects of raw and steamed extracts of Panax notoginseng and its ginsenoside constituents on human liver cancer cells.

Chinese medicine

Ding-Fung Toh, Dhavalkumar Narendrabhai Patel, Eric Chun-Yong Chan, Alvin Teo, Soek-Ying Neo, Hwee-Ling Koh

Affiliations

  1. Department of Pharmacy, Faculty of Science, National University of Singapore, 18 Science Drive 4, 117543, Singapore. [email protected].

PMID: 21255464 PMCID: PMC3037355 DOI: 10.1186/1749-8546-6-4

Abstract

BACKGROUND: Panax notoginseng is a potential source of anticancer compounds. This study aims to investigate the effects of steaming on the chemical profile of P. notoginseng and the anti-proliferative effects of P. notoginseng on liver cancer cells.

METHODS: Samples of powdered raw P. notoginseng roots were steamed for various durations. Extracts of the raw and steamed samples were subjected to ultra-high pressure liquid chromatography/mass spectrometry (UHPLC-MS) analysis for chemical profiling. The anti-proliferative effects on three human liver cancer cells, namely SNU449, SNU182 and HepG2, were evaluated using colorimetric WST-1 assay.

RESULTS: Steaming changed chromatographic and pharmacological profiles of P. notoginseng, causing differences in activities such as inhibition of cancer growth. Steamed P. notoginseng exhibited greater anti-proliferative effects against liver cancer cells (SNU449, SNU182 and HepG2) than its raw form; steaming up to 24 hours increased bioactivities. Steaming increased the concentrations of ginsenoside Rh2, Rk1, Rk3 and 20S-Rg3 and enhanced growth inhibition of liver cancer cells.

CONCLUSION: Steaming changes the chemical profile as well as anti-cancer biological activities of P. notoginseng. Steamed P. notoginseng contains potential compounds for the treatment of liver cancer.

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