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Chitwood DJ, Lusby WR, Lozano R, et al. Sterol metabolism in the nematodeCaenorhabditis elegans. Lipids. 1984;19(7):500-6doi: 10.1007/BF02534482.
Chitwood, D. J., Lusby, W. R., Lozano, R., Thompson, M. J., & Svoboda, J. A. (1984). Sterol metabolism in the nematodeCaenorhabditis elegans. Lipids, 19(7), 500-6. https://doi.org/10.1007/BF02534482
Chitwood, D J, et al. "Sterol metabolism in the nematodeCaenorhabditis elegans." Lipids vol. 19,7 (1984): 500-6. doi: https://doi.org/10.1007/BF02534482
Chitwood DJ, Lusby WR, Lozano R, Thompson MJ, Svoboda JA. Sterol metabolism in the nematodeCaenorhabditis elegans. Lipids. 1984 Jul;19(7):500-6. doi: 10.1007/BF02534482. PMID: 21344279.
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Lipids. 1984 Jul;19(7):500-6. doi: 10.1007/BF02534482.

Sterol metabolism in the nematodeCaenorhabditis elegans.

Lipids

D J Chitwood, W R Lusby, R Lozano, M J Thompson, J A Svoboda

Affiliations

  1. Insect Physiology Laboratory, Agricultural Research Service, U.S. Department of Agriculture, 20705, Beltsville, MD.

PMID: 21344279 DOI: 10.1007/BF02534482

Abstract

The metabolism of various dietary sterols and the effects of an azasteroid on sitosterol metabolism in the free-living nematodeCaenorhabditis elegans was investigated. The major unesterified sterols ofC. elegans in media supplemented with sitosterol, cholesterol or desmosterol included 7-dehydrocholesterol (66.5%, 40.5%, 31.2%, respectively), cholesterol (6.7%, 52.3%, 26.9%), lathosterol (4.4%, 3.6%, 1.7%) and 4α-methylcholest-8(14)-en-3β-ol (4.2%, 2.1%, 3.8%). Esterified sterols, representing less than 20% of the total sterols, were somewhat similar except for a significantly higher relative content of 4α-methylcholest-8(14)-en-3β-ol (23.3%, 23.4%, 10.6%). ThusC. elegans not only removes the substituent at C24 of dietary sitosterol but possesses the unusual ability to produce significant quantities of 4α-methylsterols. WhenC. elegans was propagated in medium supplemented with sitosterol plus 5 μg/ml of 25-azacoprostane hydrochloride, the azasteroid strongly interfered with reproduction and motility ofC. elegans and strongly inhibited the Δ24-sterol reductase enzyme system; excluding sitosterol, the major free sterols of azacoprostane-treatedC. elegans were cholesta-5, 7, 24-trien-3β-ol (47.9%), desmosterol (9.4%), fucosterol (2.1%) and cholesta-7,24-dien-3β-ol (2.0%). These 4 sterols are likely intermediates in the metabolism of sitosterol inC. elegans.

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