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Mol Ecol Resour. 2008 Nov;8(6):1351-3. doi: 10.1111/j.1755-0998.2008.02271.x.

Isolation and characterization of polymorphic microsatellite markers in Iberolacerta monticola, and cross-species amplification in Iberolacerta galani and Zootoca vivipara.

Molecular ecology resources

N Remón, M Vila, P Galán, H Naveira

Affiliations

  1. Departamento de Bioloxía Celular e Molecular, Universidade da Coruña, Campus A Zapateira, E-15008, A Coruña, Spain Departamento de Bioloxía Animal, Bioloxía Vexetal e Ecoloxía, Universidade da Coruña, Campus A. Zapateira, E-15008, A Coruña, Spain.

PMID: 21586043 DOI: 10.1111/j.1755-0998.2008.02271.x

Abstract

Fourteen polymorphic microsatellite loci are described for the Iberian rock lizard, Iberolacerta monticola. Genetic variation in a sample of 20 individuals from Piornedo (northwestern Spain) was quantified both by the number of alleles per locus, which ranged from six to 13, and by the expected frequency of heterozygotes under random mating (heterozygosity), which ranged from 0.761 to 0.902. Single locus and global exclusion probabilities were also computed, and indicate a high power of these markers for paternity assignments and mating system studies of I. monticola. All the analysed loci were also polymorphic in Iberolacerta galani, but only seven in Zootoca vivipara.

© 2008 The Authors. Journal compilation © 2008 Blackwell Publishing Ltd.

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