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Lazzaro G, Sharpless S, Qing W, et al. Study of nm23 gene expression in an N-methyl-N-nitrosourea transformed human breast epithelial cell line. Int J Oncol. 1997;11(4):765-9doi: 10.3892/ijo.11.4.765.
Lazzaro, G., Sharpless, S., Qing, W., Mehta, R., & Dasgupta, T. (1997). Study of nm23 gene expression in an N-methyl-N-nitrosourea transformed human breast epithelial cell line. International journal of oncology, 11(4), 765-9. https://doi.org/10.3892/ijo.11.4.765
Lazzaro, G, et al. "Study of nm23 gene expression in an N-methyl-N-nitrosourea transformed human breast epithelial cell line." International journal of oncology vol. 11,4 (1997): 765-9. doi: https://doi.org/10.3892/ijo.11.4.765
Lazzaro G, Sharpless S, Qing W, Mehta R, Dasgupta T. Study of nm23 gene expression in an N-methyl-N-nitrosourea transformed human breast epithelial cell line. Int J Oncol. 1997 Oct;11(4):765-9. doi: 10.3892/ijo.11.4.765. PMID: 21528272.
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Int J Oncol. 1997 Oct;11(4):765-9. doi: 10.3892/ijo.11.4.765.

Study of nm23 gene expression in an N-methyl-N-nitrosourea transformed human breast epithelial cell line.

International journal of oncology

G Lazzaro, S Sharpless, W Qing, R Mehta, T Dasgupta

Affiliations

  1. UNIV ILLINOIS,DEPT SURG ONCOL,COLL MED,CHICAGO,IL 60612.

PMID: 21528272 DOI: 10.3892/ijo.11.4.765

Abstract

In the present study we pinpoint the time of nm23 down-regulation during the chemical transformation of the human breast epithelial cell line HBL100. The non-malignant HBL100 was transformed by exposing it to N-methyl-N-nitrosourea (MNU). We subsequently injected the transformed cells (HBL-T-MNU) into nude mice, resulting in tumor growth. With a second passage of these tumors in mice we observed lung and extra-regional node metastases. The expression of nm23 in the non-tumorigenic HBL100 cells was compared to the tumorigenic KBL-T-MNU cells as well as to the metastatic cell line HBL-T2(MNU) derived from the tumor induced by HBL-T-MNU cells. By using immunohistochemistry and Western blot analysis we documented the downregulation of nm23 expression in the tumorigenic HBL-T-MNU and metastatic HBL-T2(MNU) cell lines, as compared to the parental line HBL100. Once downregulated, nm23 expression in this model remains constant during the subsequent progression. These results suggest that nm23 down-regulation may indeed be associated with early neoplastic transformation and is maintained throughout neoplastic progression and metastatic stage.

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