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Mitchell D, Guan X, Meltzer P. Detection of chromosome 12q amplification in sarcomas by chromosome microdissection and interphase fish. Int J Oncol. 1994;5(4):787-92doi: 10.3892/ijo.5.4.787.
Mitchell, D., Guan, X., & Meltzer, P. (1994). Detection of chromosome 12q amplification in sarcomas by chromosome microdissection and interphase fish. International journal of oncology, 5(4), 787-92. https://doi.org/10.3892/ijo.5.4.787
Mitchell, D, et al. "Detection of chromosome 12q amplification in sarcomas by chromosome microdissection and interphase fish." International journal of oncology vol. 5,4 (1994): 787-92. doi: https://doi.org/10.3892/ijo.5.4.787
Mitchell D, Guan X, Meltzer P. Detection of chromosome 12q amplification in sarcomas by chromosome microdissection and interphase fish. Int J Oncol. 1994 Oct;5(4):787-92. doi: 10.3892/ijo.5.4.787. PMID: 21559643.
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Int J Oncol. 1994 Oct;5(4):787-92. doi: 10.3892/ijo.5.4.787.

Detection of chromosome 12q amplification in sarcomas by chromosome microdissection and interphase fish.

International journal of oncology

D Mitchell, X Guan, P Meltzer

Affiliations

  1. NIH,NATL CTR HUMAN GENOME RES,CANC GENET LAB,BETHESDA,MD 20892. UNIV MICHIGAN,DEPT PEDIAT,ANN ARBOR,MI 48109.

PMID: 21559643 DOI: 10.3892/ijo.5.4.787

Abstract

We describe the microdissection of a homogeneously staining region (hsr) in the neuroblastoma cell line NGP-127 which contains the SAS gene. Fluorescence in situ hybridization (FISH) of this probe to NGP-127 cells confirms its derivation from the hsr, while FISH to normal chromosomes establishes that the hsr is composed of sequences from two segments of chromosome 12q. The hsr microdissection probe yields fluorescent signals which are sufficiently intense to readily identify amplification of homologous sequences in interphase nuclei.

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