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Leray M, Beldade R, Holbrook SJ, et al. Isolation and characterization of 13 polymorphic nuclear microsatellite primers for the widespread Indo-Pacific three-spot damselfish, Dascyllus trimaculatus, and closely related D. auripinnis. Mol Ecol Resour. 2008;9(1):213-5doi: 10.1111/j.1755-0998.2008.02380.x.
Leray, M., Beldade, R., Holbrook, S. J., Schmitt, R. J., Planes, S., & Bernardi, G. (2009). Isolation and characterization of 13 polymorphic nuclear microsatellite primers for the widespread Indo-Pacific three-spot damselfish, Dascyllus trimaculatus, and closely related D. auripinnis. Molecular ecology resources, 9(1), 213-5. https://doi.org/10.1111/j.1755-0998.2008.02380.x
Leray, Matthieu, et al. "Isolation and characterization of 13 polymorphic nuclear microsatellite primers for the widespread Indo-Pacific three-spot damselfish, Dascyllus trimaculatus, and closely related D. auripinnis." Molecular ecology resources vol. 9,1 (2009): 213-5. doi: https://doi.org/10.1111/j.1755-0998.2008.02380.x
Leray M, Beldade R, Holbrook SJ, Schmitt RJ, Planes S, Bernardi G. Isolation and characterization of 13 polymorphic nuclear microsatellite primers for the widespread Indo-Pacific three-spot damselfish, Dascyllus trimaculatus, and closely related D. auripinnis. Mol Ecol Resour. 2009 Jan;9(1):213-5. doi: 10.1111/j.1755-0998.2008.02380.x. Epub 2008 Oct 22. PMID: 21564606.
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Mol Ecol Resour. 2009 Jan;9(1):213-5. doi: 10.1111/j.1755-0998.2008.02380.x. Epub 2008 Oct 22.

Isolation and characterization of 13 polymorphic nuclear microsatellite primers for the widespread Indo-Pacific three-spot damselfish, Dascyllus trimaculatus, and closely related D. auripinnis.

Molecular ecology resources

Matthieu Leray, Ricardo Beldade, Sally J Holbrook, Russell J Schmitt, Serge Planes, Giacomo Bernardi

Affiliations

  1. Department of Ecology and Evolutionary Biology, University of California Santa Cruz, 100 Shaffer Road, Santa Cruz, CA 95060, USA, UMR 5244, CNRS-EPHE-UPVD, Centre de Biologie et d'Ecologie Tropicale et Méditerranéenne, Université de Perpignan, 66860 Perpignan Cedex, France, Coastal Research Center, Marine Science Institute, and Department of Ecology, Evolution, and Marine Biology, University of California Santa Barbara, Santa Barbara, CA 93106, USA.

PMID: 21564606 DOI: 10.1111/j.1755-0998.2008.02380.x

Abstract

A set of 13 simple sequence repeat markers was developed from D. trimaculatus genomic DNA, tested for D. auripinnis and characterized using 40 individuals per species. All the loci were polymorphic with a number of alleles ranging from three to 30. Observed heterozygosities varied from 0.23 to 0.89 for D. trimaculatus and from 0.11 to 0.85 for D. auripinnis. Early results show that these will be powerful markers for the study of ecological and evolutionary mechanism in this coral reef fish species complex.

© 2008 The Authors. Journal compilation © 2008 Blackwell Publishing Ltd.

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