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Paproski RJ, Forbrich AE, Wachowicz K, et al. Tyrosinase as a dual reporter gene for both photoacoustic and magnetic resonance imaging. Biomed Opt Express. 2011;2(4):771-80doi: 10.1364/BOE.2.000771.
Paproski, R. J., Forbrich, A. E., Wachowicz, K., Hitt, M. M., & Zemp, R. J. (2011). Tyrosinase as a dual reporter gene for both photoacoustic and magnetic resonance imaging. Biomedical optics express, 2(4), 771-80. https://doi.org/10.1364/BOE.2.000771
Paproski, Robert J, et al. "Tyrosinase as a dual reporter gene for both photoacoustic and magnetic resonance imaging." Biomedical optics express vol. 2,4 (2011): 771-80. doi: https://doi.org/10.1364/BOE.2.000771
Paproski RJ, Forbrich AE, Wachowicz K, Hitt MM, Zemp RJ. Tyrosinase as a dual reporter gene for both photoacoustic and magnetic resonance imaging. Biomed Opt Express. 2011 Mar 02;2(4):771-80. doi: 10.1364/BOE.2.000771. PMID: 21483602; PMCID: PMC3072120.
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Biomed Opt Express. 2011 Mar 02;2(4):771-80. doi: 10.1364/BOE.2.000771.

Tyrosinase as a dual reporter gene for both photoacoustic and magnetic resonance imaging.

Biomedical optics express

Robert J Paproski, Alexander E Forbrich, Keith Wachowicz, Mary M Hitt, Roger J Zemp

PMID: 21483602 PMCID: PMC3072120 DOI: 10.1364/BOE.2.000771

Abstract

Reporter genes are useful scientific tools for analyzing promoter activity, transfection efficiency, and cell migration. The current study has validated the use of tyrosinase (involved in melanin production) as a dual reporter gene for magnetic resonance and photoacoustic imaging. MCF-7 cells expressing tyrosinase appear brown due to melanin. Magnetic resonance imaging of tyrosinase-expressing MCF-7 cells in 300 μL plastic tubes displayed a 34 to 40% reduction in T1 compared to normal MCF-7 cells when cells were incubated with 250 μM ferric citrate. Photoacoustic imaging of tyrosinase-expressing MCF-7 cells in 700 μm plastic tubes displayed a 20 to 57-fold increase in photoacoustic signal compared to normal MCF-7 cells. The photoacoustic signal from tyrosinase-expressing MCF-7 cells was significantly greater than blood at 650 nm, suggesting that tyrosinase-expressing cells can be differentiated from the vasculature with in vivo photoacoustic imaging. The imaging results suggest that tyrosinase is a useful reporter gene for both magnetic resonance and photoacoustic imaging.

Keywords: (000.1430) Biology and medicine; (170.2655) Functional monitoring and imaging; (170.3880) Medical and biological imaging; (170.5120) Photoacoustic imaging

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