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Ohyashiki K, Ohyashiki J, Iwama H, et al. Telomerase reactivation in leukemia cells. Int J Oncol. 1996;8(3):417-21doi: 10.3892/ijo.8.3.417.
Ohyashiki, K., Ohyashiki, J., Iwama, H., Hayashi, S., Shay, J., & Toyama, K. (1996). Telomerase reactivation in leukemia cells. International journal of oncology, 8(3), 417-21. https://doi.org/10.3892/ijo.8.3.417
Ohyashiki, K, et al. "Telomerase reactivation in leukemia cells." International journal of oncology vol. 8,3 (1996): 417-21. doi: https://doi.org/10.3892/ijo.8.3.417
Ohyashiki K, Ohyashiki J, Iwama H, Hayashi S, Shay J, Toyama K. Telomerase reactivation in leukemia cells. Int J Oncol. 1996 Mar;8(3):417-21. doi: 10.3892/ijo.8.3.417. PMID: 21544377.
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Int J Oncol. 1996 Mar;8(3):417-21. doi: 10.3892/ijo.8.3.417.

Telomerase reactivation in leukemia cells.

International journal of oncology

K Ohyashiki, J Ohyashiki, H Iwama, S Hayashi, J Shay, K Toyama

Affiliations

  1. UNIV TEXAS,SW MED CTR,DEPT CELL BIOL & NEUROSCI,DALLAS,TX 75235.

PMID: 21544377 DOI: 10.3892/ijo.8.3.417

Abstract

We utilized fluorescent-labeled primers and modified the telomeric repeal amplification protocol (TRAP) to assess telomerase activity during the process of in vitro establishment in four different leukemia cell lines. We demonstrate that three of four leukemia cell lines had elevated telomerase activity, shortened telomeres, and the appearance of either dicentric chromosomes or acentric fragments. By contrast, one leukemia cell line (HAL-01) had high levels of telomerase activity, stable telomeres, but no obvious additional cytogenetic rearrangements. These experiments indicate that there may be several pathways involving telomerase activity in the establishment of leukemia cell lines.

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