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Moller P, Bohm M, Krugerkrasagakes S, et al. Comparison of adherent lymphokine-activated killer (a-lak) cells generated by IL-2 and IL-7 - cellular modifications induced by IL-7. Int J Oncol. 1995;7(2):383-9doi: 10.3892/ijo.7.2.383.
Moller, P., Bohm, M., Krugerkrasagakes, S., Kohlmus, C., Artuc, M., Czarnetzki, B., & Schadendorf, D. (1995). Comparison of adherent lymphokine-activated killer (a-lak) cells generated by IL-2 and IL-7 - cellular modifications induced by IL-7. International journal of oncology, 7(2), 383-9. https://doi.org/10.3892/ijo.7.2.383
Moller, P, et al. "Comparison of adherent lymphokine-activated killer (a-lak) cells generated by IL-2 and IL-7 - cellular modifications induced by IL-7." International journal of oncology vol. 7,2 (1995): 383-9. doi: https://doi.org/10.3892/ijo.7.2.383
Moller P, Bohm M, Krugerkrasagakes S, Kohlmus C, Artuc M, Czarnetzki B, Schadendorf D. Comparison of adherent lymphokine-activated killer (a-lak) cells generated by IL-2 and IL-7 - cellular modifications induced by IL-7. Int J Oncol. 1995 Aug;7(2):383-9. doi: 10.3892/ijo.7.2.383. PMID: 21552852.
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Int J Oncol. 1995 Aug;7(2):383-9. doi: 10.3892/ijo.7.2.383.

Comparison of adherent lymphokine-activated killer (a-lak) cells generated by IL-2 and IL-7 - cellular modifications induced by IL-7.

International journal of oncology

P Moller, M Bohm, S Krugerkrasagakes, C Kohlmus, M Artuc, B Czarnetzki, D Schadendorf

Affiliations

  1. HUMBOLDT UNIV BERLIN,KLINIKUM RUDOLF VIRCHOW,DEPT DERMATOL,D-13353 BERLIN,GERMANY.

PMID: 21552852 DOI: 10.3892/ijo.7.2.383

Abstract

At present, the clinical application of plastic-adherent-lymphokine-activated killer (A-LAK) cells shows limited success in the immunotherapy of patients with advanced cancer because of a low responder rate, severe side effects and failures in yielding sufficient numbers of cells for adoptive transfers. Since interleukin-7 (IL-7) is able to induce LAK activity independently of IL-2, we investigated the ability of IL-7 to improve the yield and the properties of A-LAK cells. A-LAK cells from 7 healthy donors generated in the presence of IL-2, IL-7 or combinations of IL-2 plus IL-7 (each 1000 U/ml) were compared with regard to plastic adherence, expansion rate, immunophenotype, cytokine secretion and cytotoxicity against malignant melanoma cells and non-malignant target cells. Our results demonstrate that A-LAK cells generated by a simultaneous stimulation of IL-2 plus IL-7 displayed a significantly higher expansion rate (10.7-fold vs. 9.0-fold), but showed no difference in the cytolytic activity compared to A-LAK cells generated by IL-2 alone. A-LAK cells generated by IL-7 alone demonstrated a low expansion rate (1.1-fold vs. 8.8-fold), and decreased in other properties like plastic adherence, CD56(+)/CD3(+) cell-ratio and cytolytic activity compared to A-LAK cells generated by IL-2 alone. A-LAK cells generated by IL-7 or a sequential stimulation of IL-2 and IL-7, on the other hand, exhibited a more selective cytotoxicity for malignant melanoma cells compared to the non-malignant keratinocyte target cell line (HaCaT) and normal fibroblasts. A sequential replacement of LL-2 by IL-7 might help to reduce the severe side effects of IL-2. In vivo experiments are necessary to evaluate the potential value of IL-7 in adoptive immunotherapy.

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