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Tchevkina E, Kisseljova N, Shtutman M, et al. Suppression and restoration of v-SRC expression in rsv transformed-cells after transfection with N-ras and its antagonist. Int J Oncol. 1995;7(3):453-9doi: 10.3892/ijo.7.3.453.
Tchevkina, E., Kisseljova, N., Shtutman, M., Musatkina, E., Mizenina, O., Leskov, K., Tavitian, A., & Kisseljov, F. (1995). Suppression and restoration of v-SRC expression in rsv transformed-cells after transfection with N-ras and its antagonist. International journal of oncology, 7(3), 453-9. https://doi.org/10.3892/ijo.7.3.453
Tchevkina, E, et al. "Suppression and restoration of v-SRC expression in rsv transformed-cells after transfection with N-ras and its antagonist." International journal of oncology vol. 7,3 (1995): 453-9. doi: https://doi.org/10.3892/ijo.7.3.453
Tchevkina E, Kisseljova N, Shtutman M, Musatkina E, Mizenina O, Leskov K, Tavitian A, Kisseljov F. Suppression and restoration of v-SRC expression in rsv transformed-cells after transfection with N-ras and its antagonist. Int J Oncol. 1995 Sep;7(3):453-9. doi: 10.3892/ijo.7.3.453. PMID: 21552859.
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Int J Oncol. 1995 Sep;7(3):453-9. doi: 10.3892/ijo.7.3.453.

Suppression and restoration of v-SRC expression in rsv transformed-cells after transfection with N-ras and its antagonist.

International journal of oncology

E Tchevkina, N Kisseljova, M Shtutman, E Musatkina, O Mizenina, K Leskov, A Tavitian, F Kisseljov

Affiliations

  1. CTR CANC RES,INST CARCINOGENESIS,MOSCOW,RUSSIA. INSERM,U248,F-75010 PARIS,FRANCE.

PMID: 21552859 DOI: 10.3892/ijo.7.3.453

Abstract

Previously, it was shown that hamster cells transformed by Rous Sarcoma Virus (RSV) exhibited a decreased expression of the RSV products (including the pp60 src oncogene) when these cells were supertransfected with the N-ras oncogene. To assess the responsibility of the activated N-ras in the modulation of the RSV viral products, a strategy based on two ras antagonists was used; i.e. i) a rap1A/K-rev1 expression vector known for its capacity to revert the K-ras induced transformed phenotype and ii) a plasmid containing antisense N-ras sequence. We present data showing only the plasmid construct containing the N-ras antisense sequense could inhibit expression or N-ras and, at the same time, restore the expression of v-src, up to a level comparable to that of the parental cells. Our results support the idea that some biological switches, triggered and activated through the N-ras oncogene pathway, might modulate the promoter activity of the RSV LTR.

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