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Barclay JJ, Morosi LG, Vanrell MC, et al. Trypanosoma cruzi Coexpressing Ornithine Decarboxylase and Green Fluorescence Proteins as a Tool to Study the Role of Polyamines in Chagas Disease Pathology. Enzyme Res. 2011;2011:657460doi: 10.4061/2011/657460.
Barclay, J. J., Morosi, L. G., Vanrell, M. C., Trejo, E. C., Romano, P. S., & Carrillo, C. (2011). Trypanosoma cruzi Coexpressing Ornithine Decarboxylase and Green Fluorescence Proteins as a Tool to Study the Role of Polyamines in Chagas Disease Pathology. Enzyme research, 2011657460. https://doi.org/10.4061/2011/657460
Barclay, Jeremías José, et al. "Trypanosoma cruzi Coexpressing Ornithine Decarboxylase and Green Fluorescence Proteins as a Tool to Study the Role of Polyamines in Chagas Disease Pathology." Enzyme research vol. 2011 (2011): 657460. doi: https://doi.org/10.4061/2011/657460
Barclay JJ, Morosi LG, Vanrell MC, Trejo EC, Romano PS, Carrillo C. Trypanosoma cruzi Coexpressing Ornithine Decarboxylase and Green Fluorescence Proteins as a Tool to Study the Role of Polyamines in Chagas Disease Pathology. Enzyme Res. 2011;2011:657460. doi: 10.4061/2011/657460. Epub 2011 Jun 01. PMID: 21687606; PMCID: PMC3112526.
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Enzyme Res. 2011;2011:657460. doi: 10.4061/2011/657460. Epub 2011 Jun 01.

Trypanosoma cruzi Coexpressing Ornithine Decarboxylase and Green Fluorescence Proteins as a Tool to Study the Role of Polyamines in Chagas Disease Pathology.

Enzyme research

Jeremías José Barclay, Luciano Gastón Morosi, María Cristina Vanrell, Edith Corina Trejo, Patricia Silvia Romano, Carolina Carrillo

Affiliations

  1. Fundación Instituto Leloir-(FIL-IIBBA-) CONICET and Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, 1428 Buenos Aires, Argentina.

PMID: 21687606 PMCID: PMC3112526 DOI: 10.4061/2011/657460

Abstract

Polyamines are essential for Trypanosoma cruzi, the causative agent of Chagas disease. As T. cruzi behaves as a natural auxotrophic organism, it relies on host polyamines biosynthesis. In this paper we obtained a double-transfected T. cruzi parasite that expresses the green fluorescent protein (GFP) and a heterologous ornithine decarboxylase (ODC), used itself as a novel selectable marker. These autotrophic and fluorescent parasites were characterized; the ODC presented an apparent Km for ornithine of 0.51 ± 0.16 mM and an estimated V(max) value of 476.2 nmoles/h/mg of protein. These expressing ODC parasites showed higher metacyclogenesis capacity than the auxotrophic counterpart, supporting the idea that polyamines are engaged in this process. This double-transfected T. cruzi parasite results in a powerful tool-easy to follow by its fluorescence-to study the role of polyamines in Chagas disease pathology and in related processes such as parasite survival, invasion, proliferation, metacyclogenesis, and tissue spreading.

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