Display options
Cite
Dubra A, Sulai Y, Norris JL, et al. Noninvasive imaging of the human rod photoreceptor mosaic using a confocal adaptive optics scanning ophthalmoscope. Biomed Opt Express. 2011;2(7):1864-76doi: 10.1364/BOE.2.001864.
Dubra, A., Sulai, Y., Norris, J. L., Cooper, R. F., Dubis, A. M., Williams, D. R., & Carroll, J. (2011). Noninvasive imaging of the human rod photoreceptor mosaic using a confocal adaptive optics scanning ophthalmoscope. Biomedical optics express, 2(7), 1864-76. https://doi.org/10.1364/BOE.2.001864
Dubra, Alfredo, et al. "Noninvasive imaging of the human rod photoreceptor mosaic using a confocal adaptive optics scanning ophthalmoscope." Biomedical optics express vol. 2,7 (2011): 1864-76. doi: https://doi.org/10.1364/BOE.2.001864
Dubra A, Sulai Y, Norris JL, Cooper RF, Dubis AM, Williams DR, Carroll J. Noninvasive imaging of the human rod photoreceptor mosaic using a confocal adaptive optics scanning ophthalmoscope. Biomed Opt Express. 2011 Jul 01;2(7):1864-76. doi: 10.1364/BOE.2.001864. Epub 2011 Jun 08. PMID: 21750765; PMCID: PMC3130574.
Copy Download .nbib Format: NLM
Share it on

Biomed Opt Express. 2011 Jul 01;2(7):1864-76. doi: 10.1364/BOE.2.001864. Epub 2011 Jun 08.

Noninvasive imaging of the human rod photoreceptor mosaic using a confocal adaptive optics scanning ophthalmoscope.

Biomedical optics express

Alfredo Dubra, Yusufu Sulai, Jennifer L Norris, Robert F Cooper, Adam M Dubis, David R Williams, Joseph Carroll

PMID: 21750765 PMCID: PMC3130574 DOI: 10.1364/BOE.2.001864

Abstract

The rod photoreceptors are implicated in a number of devastating retinal diseases. However, routine imaging of these cells has remained elusive, even with the advent of adaptive optics imaging. Here, we present the first in vivo images of the contiguous rod photoreceptor mosaic in nine healthy human subjects. The images were collected with three different confocal adaptive optics scanning ophthalmoscopes at two different institutions, using 680 and 775 nm superluminescent diodes for illumination. Estimates of photoreceptor density and rod:cone ratios in the 5°-15° retinal eccentricity range are consistent with histological findings, confirming our ability to resolve the rod mosaic by averaging multiple registered images, without the need for additional image processing. In one subject, we were able to identify the emergence of the first rods at approximately 190 μm from the foveal center, in agreement with previous histological studies. The rod and cone photoreceptor mosaics appear in focus at different retinal depths, with the rod mosaic best focus (i.e., brightest and sharpest) being at least 10 μm shallower than the cones at retinal eccentricities larger than 8°. This study represents an important step in bringing high-resolution imaging to bear on the study of rod disorders.

Keywords: (110.1080) Active or adaptive optics; (170.1610) Clinical applications; (170.3880) Medical and biological imaging; (170.4470) Ophthalmology; (330.5310) Vision; photoreceptors

References

  1. J Vis. 2002;2(5):404-12 - PubMed
  2. Vision Res. 2008 Nov;48(26):2564-8 - PubMed
  3. Surv Ophthalmol. 2006 May-Jun;51(3):232-58 - PubMed
  4. Invest Ophthalmol Vis Sci. 2003 Oct;44(10):4580-92 - PubMed
  5. Mol Vis. 1999 Nov 03;5:31 - PubMed
  6. J Opt Soc Am A Opt Image Sci Vis. 2007 May;24(5):1358-63 - PubMed
  7. Biomed Opt Express. 2011 Jun 1;2(6):1757-68 - PubMed
  8. Invest Ophthalmol Vis Sci. 1993 Apr;34(5):1659-76 - PubMed
  9. Orphanet J Rare Dis. 2007 Feb 01;2:7 - PubMed
  10. Vision Res. 2010 Sep 15;50(19):1989-99 - PubMed
  11. Invest Ophthalmol Vis Sci. 2006 May;47(5):2080-92 - PubMed
  12. Invest Ophthalmol Vis Sci. 1995 Jan;36(1):200-5 - PubMed
  13. Opt Express. 2008 May 26;16(11):8126-43 - PubMed
  14. Invest Ophthalmol Vis Sci. 2009 Apr;50(4):1838-47 - PubMed
  15. J Opt Soc Am A Opt Image Sci Vis. 1997 Nov;14(11):2884-92 - PubMed
  16. J Opt Soc Am A Opt Image Sci Vis. 2007 May;24(5):1250-65 - PubMed
  17. Br J Ophthalmol. 1984 Aug;68(8):561-9 - PubMed
  18. Proc Natl Acad Sci U S A. 2009 Dec 8;106(49):20948-53 - PubMed
  19. Proc Natl Acad Sci U S A. 2004 Jun 1;101(22):8461-6 - PubMed
  20. Vision Res. 2010 Apr 7;50(8):810-7 - PubMed
  21. J Neurosci. 1996 Apr 1;16(7):2251-60 - PubMed
  22. J Comp Neurol. 1990 Feb 22;292(4):497-523 - PubMed
  23. Vision Res. 2001;41(10-11):1291-306 - PubMed
  24. Invest Ophthalmol Vis Sci. 2000 Jul;41(8):2015-8 - PubMed
  25. Opt Lett. 1987 Apr 1;12(4):227-9 - PubMed
  26. Invest Ophthalmol Vis Sci. 1996 Jun;37(7):1236-49 - PubMed
  27. J Opt Soc Am A Opt Image Sci Vis. 2007 May;24(5):1438-47 - PubMed
  28. Vision Res. 1988;28(3):433-54 - PubMed
  29. Appl Opt. 1992 Jul 1;31(19):3594-600 - PubMed
  30. J Opt Soc Am A Opt Image Sci Vis. 2008 Dec;25(12):3021-9 - PubMed
  31. Arch Ophthalmol. 2000 Feb;118(2):235-45 - PubMed
  32. Vis Neurosci. 1992 Aug;9(2):169-80 - PubMed
  33. Opt Lett. 2011 Jan 1;36(1):31-3 - PubMed
  34. Invest Ophthalmol Vis Sci. 2008 Feb;49(2):713-9 - PubMed
  35. Invest Ophthalmol Vis Sci. 2007 May;48(5):2297-303 - PubMed
  36. Invest Ophthalmol Vis Sci. 1992 Jan;33(1):1-17 - PubMed
  37. Nature. 1999 Feb 11;397(6719):520-2 - PubMed
  38. Ophthalmology. 2006 Jun;113(6):1019.e1 - PubMed
  39. Invest Ophthalmol Vis Sci. 2007 Jul;48(7):3283-91 - PubMed
  40. Arch Ophthalmol. 1986 Jul;104(7):1013-20 - PubMed
  41. Biomed Opt Express. 2011 Mar 01;2(4):748-63 - PubMed
  42. J Physiol. 1983 Oct;343:577-92 - PubMed
  43. Opt Express. 2007 Oct 17;15(21):13731-44 - PubMed
  44. Vision Res. 1975 Aug-Sep;15:1005-9 - PubMed
  45. Opt Express. 2010 Mar 1;18(5):5257-70 - PubMed
  46. Invest Ophthalmol Vis Sci. 1993 Nov;34(12):3278-96 - PubMed
  47. Vis Neurosci. 1990 Dec;5(6):511-23 - PubMed
  48. Opt Express. 2009 Oct 26;17(22):19382-400 - PubMed

Publication Types

Grant support