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Tessema M, Csöregi E, Ruzgas T, et al. Oligosaccharide dehydrogenase-modified graphite electrodes for the amperometric determination of sugars in a flow injection system. Anal Chem. 1997;69(19):4039-44doi: 10.1021/ac970127f.
Tessema, M., Csöregi, E., Ruzgas, T., Kenausis, G., Solomon, T., & Gorton, L. (1997). Oligosaccharide dehydrogenase-modified graphite electrodes for the amperometric determination of sugars in a flow injection system. Analytical chemistry, 69(19), 4039-44. https://doi.org/10.1021/ac970127f
Tessema, M, et al. "Oligosaccharide dehydrogenase-modified graphite electrodes for the amperometric determination of sugars in a flow injection system." Analytical chemistry vol. 69,19 (1997): 4039-44. doi: https://doi.org/10.1021/ac970127f
Tessema M, Csöregi E, Ruzgas T, Kenausis G, Solomon T, Gorton L. Oligosaccharide dehydrogenase-modified graphite electrodes for the amperometric determination of sugars in a flow injection system. Anal Chem. 1997 Oct 01;69(19):4039-44. doi: 10.1021/ac970127f. PMID: 21639213.
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Anal Chem. 1997 Oct 01;69(19):4039-44. doi: 10.1021/ac970127f.

Oligosaccharide dehydrogenase-modified graphite electrodes for the amperometric determination of sugars in a flow injection system.

Analytical chemistry

M Tessema, E Csöregi, T Ruzgas, G Kenausis, T Solomon, L Gorton

Affiliations

  1. Department of Chemistry, Addis Ababa University, P.O. Box 1176, Addis Ababa, Ethiopia, Department of Analytical Chemistry, University of Lund, P.O. Box 124, SE-221 00, Lund, Sweden, Enzyme Chemistry Laboratory, Institute of Biochemistry, Mokslininku 12, LT-2600 Vilnius, Lithuania, and Department of Chemical Engineering, The University of Texas at Austin, Austin, Texas 78712-1062.

PMID: 21639213 DOI: 10.1021/ac970127f

Abstract

Enzyme electrodes for the determination of sugars based on solid graphite electrodes modified with oligosaccharide dehydrogenase "wired" with an osmium-based one-electron (no proton) acceptor redox hydrogel were studied as sensors in a flow injection system. The enzyme and a poly(1-vinylimidazole) (PVI) where every tenth mer is complexed with osmium (4,4'-dimethylbpy)(2)Cl, (denoted PVI(10)dmeOs) were cross-linked with poly(ethylene glycol) (diglycidyl) ether. The electrodes were active for l-arabinose, d-xylose, d-galactose, d-fructose, d-glucose, d-mannose, cellobiose, lactose, maltose, and maltooligosaccharides up to a degree of polymerization of 7. The highest relative response found was for glucose (100%) followed by maltose (40.6%) and lactose (40.6%). Fructose and isomaltotriose gave the lowest responses (<1%). Calibration curves for glucose were strictly linear in the range between 30 and 500 μM with sensitivity and apparent Michaelis-Menten constant (K(m)(app)) of 23.0 ± 1.4 μA mM(-)(1)cm(-)(2) and 4.26 ± 0.95 mM, respectively.

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