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Schmid K, Strutz J, Gleich O, et al. Localization of gentamicin uptake in the acutely isolated inner ear of the rat. Int J Physiol Pathophysiol Pharmacol. 2011;3(2):71-87
Schmid, K., Strutz, J., Gleich, O., & Kwok, P. (2011). Localization of gentamicin uptake in the acutely isolated inner ear of the rat. International journal of physiology, pathophysiology and pharmacology, 3(2), 71-87.
Schmid, Katharina, et al. "Localization of gentamicin uptake in the acutely isolated inner ear of the rat." International journal of physiology, pathophysiology and pharmacology vol. 3,2 (2011): 71-87.
Schmid K, Strutz J, Gleich O, Kwok P. Localization of gentamicin uptake in the acutely isolated inner ear of the rat. Int J Physiol Pathophysiol Pharmacol. 2011;3(2):71-87. Epub 2011 Mar 28. PMID: 21760966; PMCID: PMC3134002.
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Int J Physiol Pathophysiol Pharmacol. 2011;3(2):71-87. Epub 2011 Mar 28.

Localization of gentamicin uptake in the acutely isolated inner ear of the rat.

International journal of physiology, pathophysiology and pharmacology

Katharina Schmid, Jürgen Strutz, Otto Gleich, Pingling Kwok

Affiliations

  1. Department of Otolaryngology - Head and Neck Surgery, University of Regensburg Franz-Josef-Strauß-Allee 11, D-93042 Regensburg, Germany.

PMID: 21760966 PMCID: PMC3134002

Abstract

The quantitative analysis of fluorescence in frozen sections of rat inner ears exposed to Texas Red conjugated gentamicin revealed distinct gradients of gentamicin fluorescence. At 500 µg/ml gentamicin fluorescence occurred in inner and outer hair cells, the interdental cell region, the spiral limbus below the interdental cells, the nerve fiber bundle in the spiral lamina, the inner sulcus cells and the dorsal region of the spiral ligament. No gentamicin fluorescence was observed in the Hensen / Claudius cells, the ventral region of the spiral ligament, the stria vascularis and the spiral ganglion. In the vestibule only the hair cell epithelium and the transitional cells of the saccule showed gentamicin fluorescence while no gentamicin fluorescence was found in hair cell epithelia and transitional cells of utricle and ampule, nerve fibers below hair cell epithelia of saccule, utricle and ampule and in dark cells. The gentamicin flurescence increased at higher concentrations. Gentamicin exposure led to more pronounced gentamicin fluorescence in the cochlea compared to the vestibule. Based on the predominant gentamicin fluorescence in the hair cell - limbus region of the cochlea at a low dose we propose that gentamicin may interact with the K(+)-flow from the inner hair cells back to the scala media.

Keywords: Gentamicin; Menière's disease; cochlea; rat; vestibule

References

  1. Laryngol Rhinol Otol (Stuttg). 1977 May;56(5):409-14 - PubMed
  2. Am J Physiol Cell Physiol. 2007 Oct;293(4):C1187-208 - PubMed
  3. Acta Otolaryngol Suppl. 1957;132:1-42 - PubMed
  4. J Assoc Res Otolaryngol. 2003 Jun;4(2):196-218 - PubMed
  5. Cell Tissue Res. 2000 Oct;302(1):1-10 - PubMed
  6. Biophys J. 2008 Apr 1;94(7):2639-53 - PubMed
  7. Laryngoscope. 2004 Apr;114(4):694-7 - PubMed
  8. Acta Otolaryngol. 1965 Jul-Aug;60:113-20 - PubMed
  9. Pflugers Arch. 2006 Oct;453(1):11-22 - PubMed
  10. Hear Res. 1980 Jul;3(1):17-25 - PubMed
  11. Hear Res. 1998 Apr;118(1-2):1-12 - PubMed
  12. Hear Res. 1987 Dec 31;31(3):253-65 - PubMed
  13. Hear Res. 1985;20(3):233-44 - PubMed
  14. J Otolaryngol. 1976 Apr;6(2):135-56 - PubMed
  15. Hear Res. 2000 May;143(1-2):147-61 - PubMed
  16. Hear Res. 2002 Oct;172(1-2):127-36 - PubMed
  17. Eur Arch Otorhinolaryngol. 1998;255(3):127-31 - PubMed
  18. J Physiol. 2006 Oct 1;576(Pt 1):11-21 - PubMed
  19. Physiol Res. 2009;58(6):895-902 - PubMed
  20. HNO. 1993 Jun;41(6):296-300 - PubMed
  21. Acta Otolaryngol. 1998 Jul;118(4):530-3 - PubMed
  22. Hear Res. 2010 Mar;261(1-2):9-21 - PubMed
  23. Acta Otolaryngol Suppl. 1998;538:32-5 - PubMed
  24. Otol Neurotol. 2002 Nov;23(6):967-74 - PubMed
  25. J Assoc Res Otolaryngol. 2003 Dec;4(4):565-78 - PubMed
  26. Acta Otolaryngol Suppl. 1997;526:58-66 - PubMed
  27. Otol Neurotol. 2001 Mar;22(2):210-4 - PubMed
  28. Hear Res. 2006 Mar;213(1-2):64-78 - PubMed
  29. Ann Otolaryngol Chir Cervicofac. 1981;98(1-2):27-34 - PubMed
  30. Arch Toxicol Suppl. 1985;8:221-39 - PubMed
  31. Eur Arch Otorhinolaryngol. 1997;254(1):9-14 - PubMed
  32. Hear Res. 1999 Oct;136(1-2):139-50 - PubMed
  33. HNO. 2008 May;56(5):553-64; quiz 565-6 - PubMed
  34. Auris Nasus Larynx. 2004 Dec;31(4):383-8 - PubMed
  35. Nature. 1983 Jan 27;301(5898):334-6 - PubMed
  36. Laryngoscope. 2007 Jul;117(7):1191-8 - PubMed
  37. Acta Otolaryngol. 2006 Oct;126(10):1030-5 - PubMed
  38. Laryngoscope. 2000 Feb;110(2 Pt 1):236-40 - PubMed
  39. J Neurosci. 2002 Nov 1;22(21):9643-50 - PubMed
  40. Am J Otolaryngol. 1985 Sep-Oct;6(5):358-67 - PubMed
  41. Arch Otorhinolaryngol. 1978 Sep 28;221(2):149-52 - PubMed
  42. Proc R Soc Med. 1938 Sep;31(11):1317-36 - PubMed
  43. J Neurosci. 2010 Jan 27;30(4):1539-50 - PubMed
  44. Hear Res. 1995 Oct;90(1-2):149-57 - PubMed
  45. HNO. 2003 Nov;51(11):898-902 - PubMed
  46. Nature. 2004 Jun 17;429(6993):766-70 - PubMed
  47. Laryngoscope. 2001 Nov;111(11 Pt 1):2046-9 - PubMed
  48. Hear Res. 1996 Oct;100(1-2):80-100 - PubMed
  49. J Assoc Res Otolaryngol. 2009 Jun;10(2):205-19 - PubMed
  50. J Assoc Res Otolaryngol. 2003 Jun;4(2):176-95 - PubMed
  51. Hear Res. 2007 Aug;230(1-2):43-52 - PubMed
  52. Hear Res. 2008 Feb;236(1-2):52-60 - PubMed
  53. Am J Otolaryngol. 1990 Mar-Apr;11(2):99-111 - PubMed
  54. J Assoc Res Otolaryngol. 2007 Dec;8(4):497-508 - PubMed
  55. Otolaryngol Head Neck Surg. 1995 Sep;113(3):181-5 - PubMed
  56. Ann Otol Rhinol Laryngol Suppl. 1983 Sep-Oct;106:1-20 - PubMed

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