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Lim KY, Souckova-Skalicka K, Sarasan V, et al. A genetic appraisal of a new synthetic Nicotiana tabacum (Solanaceae) and the Kostoff synthetic tobacco. Am J Bot. 2006;93(6):875-83doi: 10.3732/ajb.93.6.875.
Lim, K. Y., Souckova-Skalicka, K., Sarasan, V., Clarkson, J. J., Chase, M. W., Kovarik, A., & Leitch, A. R. (2006). A genetic appraisal of a new synthetic Nicotiana tabacum (Solanaceae) and the Kostoff synthetic tobacco. American journal of botany, 93(6), 875-83. https://doi.org/10.3732/ajb.93.6.875
Lim, K Yoong, et al. "A genetic appraisal of a new synthetic Nicotiana tabacum (Solanaceae) and the Kostoff synthetic tobacco." American journal of botany vol. 93,6 (2006): 875-83. doi: https://doi.org/10.3732/ajb.93.6.875
Lim KY, Souckova-Skalicka K, Sarasan V, Clarkson JJ, Chase MW, Kovarik A, Leitch AR. A genetic appraisal of a new synthetic Nicotiana tabacum (Solanaceae) and the Kostoff synthetic tobacco. Am J Bot. 2006 Jun;93(6):875-83. doi: 10.3732/ajb.93.6.875. PMID: 21642150.
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Am J Bot. 2006 Jun;93(6):875-83. doi: 10.3732/ajb.93.6.875.

A genetic appraisal of a new synthetic Nicotiana tabacum (Solanaceae) and the Kostoff synthetic tobacco.

American journal of botany

K Yoong Lim, Kamila Souckova-Skalicka, Viswambharan Sarasan, James J Clarkson, Mark W Chase, Ales Kovarik, Andrew R Leitch

Affiliations

  1. School of Biological and Chemical Sciences, Queen Mary, University of London, E1 4NS, UK;

PMID: 21642150 DOI: 10.3732/ajb.93.6.875

Abstract

Polyploids have significantly influenced angiosperm evolution. Understanding the genetic consequences of polyploidy is advanced by studies on synthetic allopolyploids that mimic natural species. In Nicotiana, Burk (1973) and Kostoff (1938) generated synthetic tobacco (N. tabacum) using the parents ♀N. sylvestris × ♂N. tomentosiformis. We previously reported rapid genetic changes in the Burk material. Kostoff's material has 24 chromosomes of N. sylvestris origin (S-genome), 24 of N. tomentosiformis origin (T-genome), and a large intergenomic translocation, but not an additive distribution of ribosomal DNA (rDNA) families as expected from the parental contribution. Our new synthetic tobacco lines TR1 and TR2 are chromosomally balanced with no intergenomic translocations and are either sterile or have highly reduced fertility, supporting the nuclear cytoplasmic hypothesis that allopolyploid fertility is enhanced by intergenomic translocations. Two plants of TR1 (TR1-A, TR1-B) have the expected number, structure, and chromosomal distribution of rDNA families, in contrast to Burk's and Kostoff's synthetic tobaccos and to synthetic polyploids of Arabidopsis. Perhaps allopolyploids must pass through meiosis before genetic changes involving rDNA become apparent, or the genetic changes may occur stochastically in different synthetic allopolyploids. The lack of fertility in the first generation of our synthetic tobacco lines may have uses in biopharmacy.

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