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Matic S, Stanic S, Bogojevic D, et al. Genotoxic potential of Cotinus coggygria Scop. (Anacardiaceae) stem extract in vivo. Genet Mol Biol. 2011;34(2):298-303doi: 10.1590/s1415-47572011005000001.
Matic, S., Stanic, S., Bogojevic, D., Solujic, S., Grdovic, N., Vidakovic, M., & Mihailovic, M. (2011). Genotoxic potential of Cotinus coggygria Scop. (Anacardiaceae) stem extract in vivo. Genetics and molecular biology, 34(2), 298-303. https://doi.org/10.1590/s1415-47572011005000001
Matic, Sanja, et al. "Genotoxic potential of Cotinus coggygria Scop. (Anacardiaceae) stem extract in vivo." Genetics and molecular biology vol. 34,2 (2011): 298-303. doi: https://doi.org/10.1590/s1415-47572011005000001
Matic S, Stanic S, Bogojevic D, Solujic S, Grdovic N, Vidakovic M, Mihailovic M. Genotoxic potential of Cotinus coggygria Scop. (Anacardiaceae) stem extract in vivo. Genet Mol Biol. 2011 Apr;34(2):298-303. doi: 10.1590/s1415-47572011005000001. Epub 2011 Apr 01. PMID: 21734833; PMCID: PMC3115326.
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Genet Mol Biol. 2011 Apr;34(2):298-303. doi: 10.1590/s1415-47572011005000001. Epub 2011 Apr 01.

Genotoxic potential of Cotinus coggygria Scop. (Anacardiaceae) stem extract in vivo.

Genetics and molecular biology

Sanja Matic, Snezana Stanic, Desanka Bogojevic, Slavica Solujic, Nevena Grdovic, Melita Vidakovic, Mirjana Mihailovic

Affiliations

  1. Department of Biology and Ecology, Faculty of Science, University of Kragujevac, Serbia.

PMID: 21734833 PMCID: PMC3115326 DOI: 10.1590/s1415-47572011005000001

Abstract

The intention was to evaluate the possible in vivo genotoxic potential in different cell-types, of a methanol extract obtained from the plant stem of Cotinus coggygria Scop., using the sex-linked recessive lethal (or SLRL) test and alkaline comet assay. The SLRL test, revealed the genotoxic effect of this extract in postmeiotic and premeiotic germ-cell lines. The comet assay was carried out on rat liver and bone marrow at 24 and 72 h after intraperitoneal administration. For genotoxic evaluation, three concentrations of the extract were tested, viz., 500, 1000 and 2000 mg/kg body weight (bw), based on the solubility limit of the extract in saline. Comet tail moment and total scores in the group treated with 500 mg/kg bw, 24 and 72 h after treatment, were not significantly different from the control group, whereas in the groups of animals, under the same conditions, but with 1000 and 2000 mg/kg bw of the extract, scores were statistically so. A slight decrease in the comet score and tail moment observed in all the doses in the 72 h treatment, gave to understand that DNA damage induced by Cotinus coggygria extract decreased with time. The results of both tests revealed the genotoxic effect of Cotinus coggygria under our experimental conditions.

Keywords: Cotinus coggygria; SLRL; comet assay; extract; genotoxic effect

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