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van Rodijnen NM, Pieters M, Hoop S, et al. Data-driven compensation for flow cytometry of solid tissues. Adv Bioinformatics. 2011;2011:184731doi: 10.1155/2011/184731.
van Rodijnen, N. M., Pieters, M., Hoop, S., & Nap, M. (2011). Data-driven compensation for flow cytometry of solid tissues. Advances in bioinformatics, 2011184731. https://doi.org/10.1155/2011/184731
van Rodijnen, Nickolaas Maria, et al. "Data-driven compensation for flow cytometry of solid tissues." Advances in bioinformatics vol. 2011 (2011): 184731. doi: https://doi.org/10.1155/2011/184731
van Rodijnen NM, Pieters M, Hoop S, Nap M. Data-driven compensation for flow cytometry of solid tissues. Adv Bioinformatics. 2011;2011:184731. doi: 10.1155/2011/184731. Epub 2011 Sep 06. PMID: 21912544; PMCID: PMC3170795.
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Adv Bioinformatics. 2011;2011:184731. doi: 10.1155/2011/184731. Epub 2011 Sep 06.

Data-driven compensation for flow cytometry of solid tissues.

Advances in bioinformatics

Nickolaas Maria van Rodijnen, Math Pieters, Sjack Hoop, Marius Nap

Affiliations

  1. Department of Pathology, Atrium Medical Centre, P.O. Box 4446, 6401 CX Heerlen, The Netherlands.

PMID: 21912544 PMCID: PMC3170795 DOI: 10.1155/2011/184731

Abstract

Propidium Iodide is a fluorochrome that is used to measure the DNA content of individual cells, taken from solid tissues, with a flow cytometer. Compensation for spectral cross-over of this fluorochrome still leads to compensation results that are depending on operator experience. We present a data-driven compensation (DDC) algorithm that is designed to automatically compensate combined DNA phenotype flow cytometry acquisitions. The generated compensation values of the DDC algorithm are validated by comparison with manually determined compensation values. The results show that (1) compensation of two-color flow cytometry leads to comparable results using either manual compensation or the DDC method; (2) DDC can calculate sample-specific compensation trace lines; (3) the effects of two different approaches to calculate compensation values can be visualized within one sample. We conclude that the DDC algorithm contributes to the standardization of compensation for spectral cross-over in flow cytometry of solid tissues.

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