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Ir Vet J. 2006 Mar 01;59(3):148-52. doi: 10.1186/2046-0481-59-3-148.

Isolation of avian influenza virus (H9N2) from emu in China.

Irish veterinary journal

Wenhua Kang, Wanyong Pang, Junfeng Hao, Deming Zhao

Affiliations

  1. National Animal TSE Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing, 100094, People's Republic of China.

PMID: 21851680 PMCID: PMC3113888 DOI: 10.1186/2046-0481-59-3-148

Abstract

This is the first reported isolation of avian influenza virus (AIV) from emu in China. An outbreak of AIV infection occurred at an emu farm that housed 40 four-month-old birds. Various degrees of haemorrhage were discovered in the tissues of affected emus. Cell degeneration and necrosis were observed microscopically. Electron microscopy revealed round or oval virions with a diameter of 80 nm to 120 nm, surrounded by an envelope with spikes. The virus was classified as low pathogenic AIV (LPAIV), according to OIE standards. It was named A/Emu/HeNen/14/2004(H9N2)(Emu/HN/2004). The HA gene (1683bp) was amplified by RT-PCR and it was compared with other animal H9N2 AIV sequences in GenBank, the US National Institutes of Health genetic sequence database. The results suggested that Emu/HN/2004 may have come from an avian influenza virus (H9N2) from Southern China.

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