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Jezierski S, Gitlin L, Nagl S, et al. Multistep liquid-phase lithography for fast prototyping of microfluidic free-flow-electrophoresis chips. Anal Bioanal Chem. 2011;401(8):2651-6doi: 10.1007/s00216-011-5351-2.
Jezierski, S., Gitlin, L., Nagl, S., & Belder, D. (2011). Multistep liquid-phase lithography for fast prototyping of microfluidic free-flow-electrophoresis chips. Analytical and bioanalytical chemistry, 401(8), 2651-6. https://doi.org/10.1007/s00216-011-5351-2
Jezierski, Stefan, et al. "Multistep liquid-phase lithography for fast prototyping of microfluidic free-flow-electrophoresis chips." Analytical and bioanalytical chemistry vol. 401,8 (2011): 2651-6. doi: https://doi.org/10.1007/s00216-011-5351-2
Jezierski S, Gitlin L, Nagl S, Belder D. Multistep liquid-phase lithography for fast prototyping of microfluidic free-flow-electrophoresis chips. Anal Bioanal Chem. 2011 Nov;401(8):2651-6. doi: 10.1007/s00216-011-5351-2. Epub 2011 Sep 03. PMID: 21892629.
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Anal Bioanal Chem. 2011 Nov;401(8):2651-6. doi: 10.1007/s00216-011-5351-2. Epub 2011 Sep 03.

Multistep liquid-phase lithography for fast prototyping of microfluidic free-flow-electrophoresis chips.

Analytical and bioanalytical chemistry

Stefan Jezierski, Leonid Gitlin, Stefan Nagl, Detlev Belder

Affiliations

  1. Institut für Analytische Chemie, Universität Leipzig, Linnéstr 3, 04103 Leipzig, Germany.

PMID: 21892629 DOI: 10.1007/s00216-011-5351-2

Abstract

We present a fast and versatile method to produce functional micro free-flow electrophoresis chips. Microfluidic structures were generated between two glass slides applying multistep liquid-phase lithography, omitting troublesome bonding steps or cost-intensive master structures. Utilizing a novel spacer-less approach with the photodefinable polymer polyethyleneglycol dimethacrylate (PEG-DA), microfluidic devices with hydrophilic channels of only 25 μm in height were generated. The microfluidic chips feature ion-permeable segregation walls between the electrode channels and the separation bed and hydrophilic surfaces. The performance of the chip is demonstrated by free-flow electrophoretic separation of fluorescent xanthene dyes and fluorescently labeled amino acids.

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